金线莲分化芽离体培养条件的优化  

Optimization of the Conditions for In Vitro Culture of Regenerated Bud of Anoectochilus roxburghii

作　　者：杨红丽[1] 胡靖锋[1] 徐学忠[1] 和江明[1] 宋爽[2] 

机构地区：[1]云南省农业科学院园艺作物研究所,云南昆明650205 [2]云南省农业科学院行政处,云南昆明650205

出　　处：《湖南农业科学（下半月）》2014年第3期52-54,共3页

基　　金：国家大宗蔬菜产业技术体系(CARS-25-G-45);农业部公益性行业专项(201003029);云南省科技厅重点新产品开发计划(2012BB017);云省农业厅蔬菜新品种协作项目[云财农(2012)58号]

摘　　要：以金线莲分化芽为外植体,研究了在培养基中添加不同的碳源、有机物质、活性炭,以及调节培养基的pH值和培养温度对金线莲离体培养的影响。结果表明:蔗糖是金线莲离体培养的最佳碳源;培养基中添加一定量的香蕉或椰汁和2 g/L的活性炭能显著加快金线莲的生长速度,离体培养的最适pH值为5.8,最适生长温度为25℃。The regenerated buds of Anoectochilus roxburghii were taken as explants to study the influences of adding different carbon sources, organic substances and activated carbons to the culture medium and regulating the pH value and temperature of the culture medium on effect of in vitro culture ofAnoectochilus roxburghii. The results showed that sucrose is the best carbon source for in vitro culture of Anoectochilus roxburghii; adding a certain amount of banana or coconut juice and 2 g/L of activated carbon to the culture medium can significantly accelerate the growth speed ofAnoectochilus roxburghii; the optimal pH value and the optimal temperature for in vitro culture ofAnoectochilus roxburghii were 5.8 and 25 ℃, respectively.

关 键 词：金线莲 分化芽 生长 营养特性 离体培养 

分 类 号：S567.239[农业科学—中草药栽培]