黄芪多糖改善游离脂肪酸致胰岛β细胞的脂毒性  

Protective Effect of Astragalus Polysaccharides on Lipotoxicity of Free Fatty Acid to Islet β Cells

作　　者：胡阳黔[1] 李静[2] 刘坚[2] 王文峰[1] 宋杰[1] 

机构地区：[1]湖北医药学院附属东风医院消化内科,十堰442000 [2]湖北医药学院基础医学院人体解剖学教研室,十堰442000

出　　处：《华中科技大学学报（医学版）》2014年第2期181-184,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.30370673);湖北省教育厅科研项目(No.B2013120);十堰市科技局科技项目(No.ZD2012037)

摘　　要：目的探讨黄芪多糖(astragalus polysaccharides,APS)对游离脂肪酸(free fatty acid,FFA)导致的大鼠胰岛β细胞脂毒性的保护作用及机制。方法体外分离培养Wistar大鼠胰岛细胞,分为4组:对照组、APS组、FFA组、FFA+APS组。各组细胞培养72h测胰岛内三酰甘油(TG)、游离脂肪酸(FFA)含量,基础状态胰岛分泌情况,Western blot法测胰岛β细胞内总腺苷酸活化蛋白激酶(AMPK),磷酸化腺苷酸活化蛋白激酶(p-AMPK),肉毒碱软脂酰转移酶-1(CPT-1)、乙酰辅酶A羧化酶(ACC)的蛋白表达情况。结果①FFA使胰岛内TG、FFA含量增加(均P<0.01),APS使胰岛内TG、FFA含量减少(均P<0.01);②油红O染色提示FFA组胰岛内脂质蓄积增加,APS可减少脂质蓄积;③FFA使胰岛素分泌增加(P<0.01),APS减少胰岛素分泌(P<0.01);④FFA抑制p-AMPK、CPT-1、ACC表达,APS使p-AMPK、CPT-1、ACC表达增加(均P<0.01)。结论 FFA使胰岛内脂质蓄积增加,胰岛素分泌增加,形成高胰岛素血症。APS可以通过AMPK脂代谢途径减少胰岛内脂质蓄积,同时减少胰岛素分泌,减轻高胰岛素血症,从而对FFA导致的大鼠胰岛β细胞脂毒性起到保护作用。Objective To investigate the protective effect of astragalus polysaccharides（APS）on the lipotoxicity of free fatty acid（FFA）to isletβcells and its mechanism.Methods Pancreatic islet cells of Wistar rat were isolated and cultured in vitro.They were divided into four groups：control group,APS group,FFA group,FFA ＋APS group.The content of triglycerides（TG）and FFA and the insulin secretion were measured in cells cultured for 72hin each group.The expression of total AMP-activated protein kinase（AMPK）,phosphorylated adenylate-activated protein kinase（p-AMPK）,carnitine palmitoyl transferase-1（CPT-1）,acetyl CoA carboxylase（ACC）were detected by Western blot.Results ①The contents of TG and free fatty acid were increased in the FFA group（P〈0.01）but decreased in the FFA＋APS group（P 〈0.01）;②Oil Red O staining showed that lipid accumulation increased in the FFA group but reduced in the FFA＋APS group（P〈0.01）;③FFA could increase the insulin secretion（P〈0.01）,and APS could reduce the insulin secretion（P〈0.01）.④The expressions of pAMPK,CPT-1and ACC were significantly inhibited in the FFA group but conspicuously increased in the FFA＋APS group（P〈 0.01）.Conclusion FFA can increase lipid accumulation and insulin secretion in the islets and lead to hyperinsulinemia.APS can reduce lipid accumulation and insulin secretion in the islets and alleviate hyperinsulinemia through AMPK-mediated lipid metabolism pathway,and it can protect isletβcells against FFA-caused lipotoxicity.

关 键 词：黄芪多糖 游离脂肪酸 腺苷酸活化蛋白激酶 脂毒性 胰岛Β细胞 

分 类 号：R587[医药卫生—内分泌]