野生番茄耐盐性研究及其利用  

Evaluation of Salt Tolerance on Wild Tomato Species and Its Utilization

作　　者：陈火英[1] 张建华[2] 钟建江[1] 俞俊棠[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]上海交通大学植物科学系,上海201101

出　　处：《华东理工大学学报（自然科学版）》2001年第1期51-55,共5页Journal of East China University of Science and Technology

基　　金：上海市"曙光"基金项目! (98SG47)

摘　　要：将带有二叶一心的小苗扦插于附加不同浓度 Na Cl的 MS培养基中 ,研究不同番茄材料发根、植株生长以及 POD酶酶谱及其活性的变化 ,并通过花粉管导入技术将野生番茄的总 DNA导入到栽培种。研究结果表明 ,Lycopersicon pennellii LA71 6和 Lycopersicon pimpinellifoliumLA2 1 84始终具有相对高的生根率 ,在 1 50 mmol/L Na Cl胁迫 1 0 d时 ,相对发根率分别为 86.8%和1 0 0 %。参试的 4份野生种 ,在附加有 75mmol/L Na Cl的培养基上的株高和叶片数均高于对照 (无Na Cl) ,随着 Na Cl浓度的不断提高 ,株高和叶片数都下降 ,Lycopersicon pennellii LA71 6下降幅度最小。 4份野生种的耐盐性明显优于 2份栽培种 ,当 Na Cl浓度提高到 30 0 mmol/L时 ,4份野生种的鲜重可达对照的 0 .90、1 .0 7、1 .60和 1 .53倍 ,而 2份栽培种则为对照的 0 .55和 0 .33倍。当Na Cl浓度为 75mmol/L时 ,Lycopersicon peruvianum LA1 1 1和 Lycopersicon pennellii LA71 6材料 POD酶酶带数多于对照 2条和 1条 ,Lycopersicon cheesmanii LA1 66和 Lycopersicon pimpinellifoliumLA2 1 84则少于对照 1条和 3条。利用花粉管导入技术可以将野生番茄的 DNA导入到栽培番茄。The possibility of using in vitro shoot culture to evaluate salt tolerance of different tomato species was determined. Shoot with 3 leaves grew in media supplemented with 0,75,150,300,450 mmol/L NaCl, and rooting and growth were determined. The different degree of salt tolerance among species was clearly shown on the basis of the shoot rooting and growth. Studies on POD isoenzymes at 75mmol/L NaCl stress and without stress were carried out. Exogenous DNA extracted and purified from the leaves of wild tomato species was injected into the ovary of cultivated tomato about 24h after artificial self pollination. Some characters of the donor were observed in offsprings. The results obtained in this paper showed Lycopersicon pennellii LA716 was the most salt tolerant in vitro shoot culture and it could be a better system for testing and selecting for salt tolerance.

关 键 词：番茄 发根 植株生长 过氧化物酶 氯化钠胁近 耐盐性 基因转移 花粉管导入技术 

分 类 号：S641.203.4[农业科学—蔬菜学]