一个新的小麦-中间偃麦草异代换系的分子细胞学鉴定  

Identification for a New Wheat Alien Substitution Lines Derived from Th. intermedium by Molecular Cytology

作　　者：胡静[1] 李欣[2] 阎晓涛[2] 任永康[2] 郭慧娟[2] 詹海仙[2] 乔麟轶[2] 畅志坚[2] 张晓军[2] 

机构地区：[1]山西大学生物工程学院,山西太原030006 [2]山西省农业科学院作物科学研究所,山西太原030032

出　　处：《山西农业科学》2014年第5期425-427,431,共4页Journal of Shanxi Agricultural Sciences

基　　金：国家自然科学基金项目(31171839);山西省国际科技合作计划项目(2012081006-2;2013081007);山西省科技攻关项目(20130311001-5);山西省回国留学人员科研资助项目(2012-102);山西省科技创新团队建设项目(2013131025);山西省农业科学院科技攻关项目(2013YGG54)

摘　　要：以来源于中间偃麦草的八倍体小偃麦为桥梁亲本,通过回交转育的方法,将其所携带的中间偃麦草染色体转移到普通小麦中,从其BC1F6选系中选育出一个高抗小麦秆锈病、白粉病的优良品系CH08-141,并利用分子细胞学方法对其进行鉴定。以中间偃麦草基因组DNA为探针的GISH结果表明,CH08-141中包含1对来源于中间偃麦草的染色体,属于小麦-中间偃麦草异代换系;以平均分布于小麦基因组染色体上的48对SSR引物对CH08-141分子标记染色体定位,结果表明,CH08-141中的6B染色体被中间偃麦草的1对J组染色体所代换。新鉴定的异代换系含有来源于中间偃麦草的抗秆锈病和白粉病基因,是小麦抗病育种中不可多得的新抗源。New wheat germplasm CH08-141 was selected from the BC1F6 groups of Jintai 170 crossed with partial amphiploid TAI7047 derived from Th.intermedium amphiploid.CH08-141 is highly wheat stem rust and powdery mildew resistant through resistance identification.And the resistance genes in CH08-141 comes from Th.intermedium amphiploid.G enomic in situ hybridisation （GISH） of Th.intermedium amphiploid genomic DNA as probes showed that CH08-141 contains one pair of chromosomes from Th.intermedium amphiploid.48 pairs SSR primers evenly distributed on the wheat genome chromosomes were used to detect the missing wheat chromosomes in CH08-141.The results show that one pair of wheat group 6B chromosomes in CH08-141 were replaced by the Group J of Th.intermedium amphiploid.Therefore,we considered that the wheat germplasm CH08-141 is a new wheat-Th.intermedium amphiploid alien substitution line.

关 键 词：小麦 中间偃麦草 异代换系 GISH SSR 

分 类 号：S512.1[农业科学—作物学]