水通道蛋白7和8在牛早期胚胎发育过程中的表达规律  

Expression pattern of AQP7 /8 in the bovine embryo during early development

作　　者：王帅[1,2] 邓琼[1,2] 徐芳芳[1,2] 李常红[1,2] 任久生[1,2] 戴立胜[1,2] 张嘉保[1,2] 

机构地区：[1]吉林大学动物科学学院,吉林长春130062 [2]吉林大学实验动物中心,吉林长春130062

出　　处：《畜牧与兽医》2014年第5期6-9,共4页Animal Husbandry & Veterinary Medicine

基　　金：国家科技支撑计划(2011BAD19B04)

摘　　要：以牛卵母细胞为研究对象,体外成熟后孤雌激活,收集各个时期的卵母细胞和不同发育阶段的早期胚胎,提取总RNA。根据GenBank公布的mRNA序列设计引物,进行RT-qPCR,检测不同阶段AQP7与AQP8的时空表达规律,结果表明:AQP7与AQP8在不同时期卵母细胞和早期胚胎中具有相似的表达规律,在8Cell达到峰值,而到16Cell期表达量均有显著下降。在牛早期胚胎发育阻滞期(8-16Cell期),胚胎发育由母源基因主导调控转向依赖合子自身遗传物质,这期间AQP7与AQP8表达变化说明其在转变过程中具有重要作用,本试验为AQP7与AQP8在牛早期胚胎发育过程中的功能研究奠定基础。In this study, we focused on the bovine oocyte, oocytes and embryos at different developmental stages after in vitro maturation and parthenogenetic activation were collected for RNA extraction. AQPT/8 expressions at different stages were analyzed by RT-qPCR with the primers designed by Oligo 6. 0. We found that AQP7/8 had a similar expression pattern, especially in early block period （8Cell- 16Cell） , showing a significant decrease. The study provided a foundation for the function research of AQP7/8 in the early embryo develop- ment of bovines.

关 键 词：牛 早期胚胎发育 水通道蛋白 表达规律 

分 类 号：S814.6[农业科学—畜牧学]