日粮能量水平对幼龄獭兔血液生化指标及肝脏PPARγ、INSIG-2基因表达的影响  

Effect of diet energy level on blood parameters and expression of PPARγand INSIG-2 genes in liver of juvenile rabbits

作　　者：宋洪新[1] 任战军[1] 杨雪娇[1] 贺国瑞[1] 韩营[1] 常万波[1] 颉宏兵 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]甘肃康园有限公司,甘肃甘谷741200

出　　处：《西北农林科技大学学报（自然科学版）》2014年第5期11-16,21,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：陕西省农业科技攻关项目(2010K01-16)

摘　　要：【目的】研究日粮能量水平对断奶至3月龄獭兔血液生化指标以及肝脏过氧化物酶体增殖剂激活受体γ基因（PPARγ）、胰岛素诱导基因2（INSIG-2）表达的影响。【方法】选用断奶的健康獭兔160只,随机分为4组,每组40只,4组试验兔的日粮能量水平分别为9.7,10.5,11.3和12.1MJ/kg,试验分断奶~2月龄、2~3月龄2个阶段。分别于试验开始的第30,60天早晨采血,测定血液中的葡萄糖、甘油三酯、总蛋白、总胆固醇、高密度胆固醇、低密度胆固醇含量及谷丙转氨酶、谷草转氨酶、碱性磷酸酶、乳酸脱氢酶活性;并取肝脏样品,采用实时荧光定量法测定PPARγ和INSIG-2基因的表达情况。【结果】在2月龄时,日粮能量水平对獭兔血液中的总胆固醇含量和谷草转氨酶活性有极显著影响（P〈0.01）,对高密度胆固醇和低密度胆固醇含量有显著影响（P〈0.05）,对其他血液指标影响不显著。3月龄时,日粮能量水平对獭兔血液中的总蛋白质含量有极显著影响（P〈0.01）,对谷草转氨酶活性有显著影响（P〈0.05）,对其他血液指标影响不显著。在2、3月龄时,PPARγ基因均分别以11.3和12.1 MJ/kg日粮能量水平下的表达量最高,INSIG-2基因则分别以11.3和9.7MJ/kg日粮能量水平下的表达量最高。【结论】日粮能量水平对幼龄獭兔血液中总蛋白质、总胆固醇、高密度胆固醇、低密度胆固醇含量和谷草转氨酶活性有显著影响,对其他血液指标无显著影响;日粮能量水平对幼龄獭兔肝脏PPARγ、INSIG-2基因的表达量均有显著影响。[Objective] This study aimed to study the effect of diet energy level on blood parameters and expression of PPAR）, and INSIG-2 genes in liver of juvenile rabbits. [Method] 160 healthy weaning rabbits were divided them into 4 groups randomly, each group contained 40 rabbits. Diets energy levels of the 4 groups were 9.7,10.5,11.3 and 12.1 MJ/kg, respectively. The experiment had two stages, weaning-2 months and 2-3 months. [Result] Diet energy levels had extremely significant effect on the contents of TC and AST （P〈0. 01） ,significant effect on the contents of HDLC and LDLC （P〈0. 05） ,no significant effect on other parameters in blood of 2 months old rabbits. Diet energy levels had extremely significant effect on the content of TP （P〈0.01） ,significant effect on the content of AST （P〈0.05） ,and no significant effect on other parameters in blood of 3 months old rabbits. Expression levels of PPARγ gene were the highest in 2 and 3 months old rabbits with energy level of 11.3 and 12.1 MJ/kg,respectively. Expres sions of INSIG-2 gene were the highest in 2 months old rabbits with energy level of 11.3 MJ/kg and in 3 months old rabbits with energy level of 9.7 MJ/kg. [Conclusion] Diet energy levels had significant effect on the contents of TP, TC, HDLC, LDLC and AST, while no significant influence on other blood indicators of young rabbits. Effect of diet energy levels on expression of PPARγ and INSIG-2 was significantly.

关 键 词：獭兔 日粮 能量水平 血液指标 基因表达量 

分 类 号：S829.15[农业科学—畜牧学]