致血流感染非O1群非O139群霍乱弧菌的鉴定及毒力基因检测  

Identification and virulence gene detection of non-O1 and non-0139 Vibrio cholerae isolates causing septicemia

作　　者：邹玖明[1] 张爱平[2] 李智山[1] 杨燕[1] 赵建忠[1] 

机构地区：[1]襄阳市中心医院检验科,湖北襄阳441021 [2]襄阳市中心医院重症医学科,湖北襄阳441021

出　　处：《中国感染与化疗杂志》2014年第3期186-189,共4页Chinese Journal of Infection and Chemotherapy

摘　　要：目的对襄阳市中心医院分离1株疑似霍乱弧菌进行鉴定及药物敏感性试验,并检测其主要毒力基因。方法利用MicroScan WalkAway 40鉴定仪进行生化鉴定及药物敏感性试验,玻片凝集法确定其血清型别,应用PCR及测序技术分析其16SrRNA基因;PCR检测其6个毒力基因。结果经鉴定,该株疑似霍乱菌株为非O1群非O139群霍乱弧菌,经16SrRNA分析与美国国家生物技术信息中心数据库中霍乱弧菌相似性达100%。药敏试验结果显示该菌对氨苄西林、氯霉素、甲氧苄啶-磺胺甲(口恶)唑、四环素均敏感,毒力基因检测rtxC和toxR阳性,tcpAET、ctxA、hlyA、tcpACL阴性。结论该株疑似霍乱弧菌为非O1群非O139群霍乱弧菌,其致病与rtxC、toxR毒力基因有关。Objective To identify an suspected Vibrio cholerae isolated from Xiangyang Central Hospital and characterize the strain in terms of antibiotic resistance and relevant virulence genes.Methods Pathogen identification and susceptibility testing were completed with MicroScan WalkAway 40 Automated Microbiology System.Slide agglutination was used for serotyping.PCR and sequencing technology were employed for 16s RNA gene analysis.PCR technique was used to detect six major virulence genes.Results This suspected Vibrio cholerae isolate was confirmed as non-O1 and non-0139 Vibrio cholerae.Susceptibility testing results indicated that the strain was sensitive to ampicillin,chloramphenicol,trimethoprim-sulfamethoxazole,and tetracycline.16s RNA gene sequence analysis showed 100%homologous with the registered sequence in National Center for Biotechnology Information database.Virulence genes rtxC and toxR were identified.The other virulence genes such as tcpAET,ctxA,hlyA,and tcpACL were negative.Conclusions This suspected Vibrio cholerae isolate is confirmed as non-Ol and non-0139 Vibrio cholerae.The pathogenic factors may be related to the virulence genes rtxC and toxR.

关 键 词：霍乱弧菌 基因 毒力 血清型 

分 类 号：R378.3[医药卫生—病原生物学]