罗田板栗玫瑰红栗实内生细菌的分离鉴定  

Isolation and Identification of the Endophytic Bacteria from Castanea mollissima ‘Meiguihong' of Luotian County

作　　者：李琳玲[1] 华娟[1] 程华[1] 廖志琴[1] 汪玉平[1] 袁红慧[1] 程水源[1] 

机构地区：[1]黄冈师范学院经济林木种质改良与资源综合利用湖北省重点实验室,湖北黄冈438000

出　　处：《湖北农业科学》2014年第4期806-808,813,共4页Hubei Agricultural Sciences

基　　金：中央财政林业科技推广示范项目(2011BH0032);湖北省教育厅高校产学研合作资助重点项目(C2010060);湖北省教育厅中青年基金项目(Q20132909)

摘　　要：为了探明罗田板栗新品系玫瑰红耐贮藏的内在原因,评价其栗实中的内生细菌资源,用组织分离培养法从栗实中分离其内生细菌,并通过比对结构、生理生化特征及16S rDNA序列分析等方法对所分离的内生细菌进行鉴定。结果从玫瑰红板栗栗实中分离得到2株内生细菌FR1和FR2,经鉴定为水生拉恩菌(Rahnella aquatilis)和地衣芽孢杆菌(Bacillus licheniformis)。In order to explore the storage tolerance mechanism of Castanea mollissima Blume. ‘Meiguihong＇, a new variety of chestnut from Luotian, and the resources of endophytic bacteria were evaulated from its chestnut. Two kinds of pathogenic bacterium, FR1 and FR2, were isolated from the decayed seeds of Chinese chestnut. These two strains were preliminarily identified as Rahnella aquatilis and Bacillus licheniformis by comparing structure, characteristics of biochemistry and physiology, sequence of 16S rDNA.

关 键 词：罗田板栗 玫瑰红 内生细菌 分离鉴定 16S RDNA 

分 类 号：S664.2[农业科学—果树学] Q948.122.3[农业科学—园艺学]