蓖麻细胞悬浮培养技术研究  

The Cell Suspension Culture of Ricinus communis L.

作　　者：康薇[1] 郑进[1,2] 

机构地区：[1]湖北理工学院,湖北黄石435003 [2]矿区环境污染控制与修复湖北省重点实验室,湖北黄石435003

出　　处：《湖北农业科学》2014年第4期932-935,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金面上项目(41371470);湖北省自然科学基金重点项目(2011CDA037);湖北省黄石市科技计划项目(2010A1019-2)

摘　　要：生长在大冶市铜绿山矿区的野生蓖麻（Ricinus communis L.）是一种新发现的铜超富集植物。为了建立稳定、快速生长的蓖麻悬浮细胞系,以便筛选重金属抗性蓖麻细胞和培育超富集植株,以前期诱导的蓖麻胚性愈伤组织为材料,通过正交试验设计,优化悬浮细胞系的培养条件,建立蓖麻细胞悬浮系,并在优化条件下测定细胞的生长曲线和培养液pH变化。结果表明,蓖麻细胞悬浮系的最佳培养条件为接种量2.0 g/50 mL;基本培养基MS＋0.5 mg/L 6-BA＋0.2 mg/L NAA＋50 mg/L蔗糖＋350 mg/L水解酪蛋白;温度（26±2）℃,110 r/min暗培养。此时,细胞鲜重和干重增长量最高分别达到4.58 g/（50 mL·14 d）和0.49 g/（50 mL·16 d）。蓖麻悬浮细胞系生长曲线基本呈＂S＂形,每16 d为一个培养周期,其中6～14 d为快速增长期。在一个培养周期内,培养液pH表现为先降后升,然后趋于稳定。Ricimts communis was a new hyperaceumulator growing on Tonglushan copper mine in Daye city. A stable and rapid grnwth cell suspension culture line of R. communis was established to screen heavy-resistance cell and accumulate plants using embyrogenic cellus with orthogonal experimental design. The results showed that the optimal euhuring conditions were MS medium with 0.5 mg/L 6-BA ＋ 0.2 mg/L NAA ＋ 50 mg/L sucrose ＋ 350 mg/L casein hydrolyzate anti 2.0g（FW）/ 50 ml, inoculation quantity,culture temperature （26±2）℃,drak culture,110 r/rain. The best growth rate of the cells weir 4.58 g/（50 mL·14 d） and 0.49 g/（50 mL·16 d） under the optimal conditions, respectively. The growing curves of R. communis suspension cell shaped by “S” nearly. The cultutring period was 16 d with the rapid growth phase from 6 to 14 d. The pH trend of cuhurc liquid was declined first anti then increased to stable gradually during a culturing period.

关 键 词：蓖麻(Ricinus communis L.) 胚性愈伤组织 正交试验设计 悬浮细胞系 

分 类 号：S565.6[农业科学—作物学]