不同针刺刺激量对寒凝证类痛经大鼠疼痛及子宫μ受体的影响  

Effect of Different Acupuncture Stimulus on Pain and Uterus μ Receptor of Dysmenorrhea Rats with Cold Syndrome

作　　者：张玲[1] 郭盂玮[1,2] 申松希[1] 朱世鹏[1] 齐丹丹[1] 任晓暄[1,2] 赵雅芳[1,2] 嵇波[1,2,3] 朱江[1,3] 李晓泓[1,2,3] 张露芬[1,2,3] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]国家中医药管理局针灸生物学三级实验室,北京100029 [3]针灸特色疗法评价重点研究室,北京100029

出　　处：《中医药学报》2014年第2期8-12,共5页Acta Chinese Medicine and Pharmacology

基　　金：国家重点基础研究发展计划(973计划)资助项目(2012CB518506;2006CB504503);国家自然科学基金面上项目(No.30973793);教育部博士点基金项目(No.20090013110005)

摘　　要：目的:观察不同针刺刺激量对寒凝证类痛经大鼠模型的镇痛效应及子宫组织μ受体表达的影响。方法:按随机数字表法将64只雌性SD大鼠,随机分为4组,每组16只,分别为:盐水组、寒凝证类痛经模型组、刺激量A组、刺激量B组。除盐水组外均给予大鼠注射苯甲酸雌二醇和缩宫素2U/只,并于第1天至第5天放入-25℃冰柜冷冻,制备寒凝证类痛经大鼠模型;盐水组每日给予同等剂量的生理盐水。第10天注射缩宫素后,刺激量A组和刺激量B组以不同刺激量进行针刺治疗。记录疼痛即刻开始20min扭体评分和次数、子宫收缩波;以RealTime-PCR法测定子宫组织内μ受体。结果:①与盐水组比较,寒凝证类痛经模型组扭体潜伏期均明显缩短、扭体次数明显增多、扭体总分明显增多(P<0.01)。与寒凝证类痛经模型组比较,刺激量A组扭体潜伏期明显延长、扭体次数减少、扭体评分减少(P<0.01)。与刺激量A组比较,刺激量B组扭体潜伏期明显缩短、扭体次数明显增多、扭体评分明显升高(P<0.01);②与盐水组相比,寒凝证类痛经模型组子宫收缩波个数明显增加(P<0.01),子宫收缩波峰峰值显著升高(P<0.01),子宫活动度显著增加(P<0.01);与寒凝证类痛经模型组比较,刺激量A组显著减少了子宫收缩波个数(P<0.01)并降低了子宫活动度(P<0.05),并且在减少子宫收缩波个数方面优于刺激量B组(P<0.05);③与寒凝证类痛经模型组比较,刺激量A组子宫组织中μ受体表达显著增高刺激量A组(P<0.01),刺激量B组(P<0.05)。结论:刺激量A组可明显减轻寒凝证类痛经大鼠疼痛,缓解子宫痉挛性收缩,其与调节阿片受体的水平密切相关,提示刺激量A组对寒凝证类痛经大鼠具有明显的镇痛效应。Objective： To investigate the effect of different acupuncture stimulus on pains and expression of μ opioid receptor of primary dysmenorrheal model rats with cold syndrome. Methods： Female rats in anestrus were randomly divided into four groups,which included saline control group,cold syndrome of primary dysmenorrheal model control group（ model control group）,A stimuli group and B Stimuli group. Saline control group rats were injected 0. 9% Nacl. All other rats were injected Estradiol Benzoate. From the first day to the fifth day rats（ except saline control group） were put into- 25℃ freezer to make cold syndrome of primary dysmenorrheal model rats. Different amounts of acupuncture were used in A Stimuli group and B Stimuli group. BL- 420F logger of experimental system was applied to record times and intensity of uterine contraction in 20 minutes and μ- receptor in uterine was detected by RealTime- PCR. Results： ①Model control group comparing with the saline control group： writhing latency significantly decreased（ P〈0. 01）, writhing number and writhing number significantly increased（ P〈0. 01）. A Stimuli group comparing with the model control group： writhing latency significantly increased（ P〈0. 01）,writhing number and writhing number significantly decreased（ P 0. 01）. B Stimuli group comparing with A Stimuli Group writhing latency significantly decreased（ P 〈 0. 01）; ②writhing number and writhing number significantly increased（ P〈0. 01）; Model control group comparing with the saline control group： the times,peak to peak of uterine contraction and intensity of uterine contraction significantly increased（ P 〈 0. 01）,which meant the smooth muscle of uterus was induced spasmodic contraction. A Stimuli group comparing with the model control group： the times and the intensity of uterine contraction of uterine were reduced significantly（ P 〈 0. 05） and better than B Stimuli group（ P 〈 0. 05）. ③A Stimuli group increased the amount of μ op

关 键 词：寒凝类痛经大鼠模型 得气 子宫张力μ受体 

分 类 号：R245.3[医药卫生—针灸推拿学]