RP-HPLC法测定六味千骨巴布剂中柚皮苷的含量  

Determination of Naringin in Liuwei Qiangu Cataplasm by RP- HPLC

作　　者：刘世萍[1] 郭美华[1] 李小红[1] 马妍妍[1] 白建海[2] 王琪[1] 

机构地区：[1]哈尔滨医科大学附属第一医院药学部,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第一医院眼科,黑龙江哈尔滨150001

出　　处：《中医药学报》2014年第2期49-51,共3页Acta Chinese Medicine and Pharmacology

基　　金：黑龙江省中医药科研项目(ZHY12-Z155)

摘　　要：目的:建立以高效液相色谱法测定六味千骨巴布剂中柚皮苷含量的方法。方法:色谱柱为HyperClone 5u BDS C18130A柱(250mm×4.6mm,5μm),流动相为甲醇-水-冰乙酸(45:55:2),检测波长284nm,流速:1.0mL/min,柱温为28℃。结果:柚皮苷进样量在0.061 6μg^1.232μg的范围内呈良好的线性关系,r=0.999 9,平均回收率为98.34%,RSD=1.04%(n=6)。结论:该法操作简单、快速、准确,专属性强,可作为该制剂的质量控制方法。Objective： To establish a method for the determination of Naringin in Liuwei Qiangu Cataplasm by RP-HPLC. Methods： The HyperClone 5u BDS C18130A column（ 250mm × 4. 6mm,5micron） was used,and the mobile phase was methand- waters- acetic（ 45： 55： 2）. The flow rate was 1. 0mL / min; the column temperature was 28℃; the detection wavelength was 284 nm. Results： The linear range of Naringin was within 0. 061 6μg ～ 1. 232μg（ r = 0. 999 9）. The average recovery was 98. 34%,RSD = 1. 04%（ n = 6）. Conclusion： The method is simple,fast,and the result is accurate and reliable. It can be used for quality control of the Naringin in Liuwei Qiangu Cataplasm.

关 键 词：六味千骨巴布剂 反相高效液相色谱法 柚皮苷 

分 类 号：R284.1[医药卫生—中药学]