不同pH值对人胚肺二倍体成纤维细胞MRC-5生长的影响  

Effect of different pH on growth of human embryonic lung diploid fibroblasts MRC-5 cells

作　　者：陈通威 赵鹏 

机构地区：[1]山西康宝生物制品股份有限公司,长治046011

出　　处：《山西医科大学学报》2014年第5期347-349,共3页Journal of Shanxi Medical University

摘　　要：目的在不同pH值培养液中培养人胚肺二倍体成纤维细胞(MRC-5),观察其生长情况,选出最有利于MRC-5细胞生长的pH值范围。方法采用细胞培养技术,分别在pH值为6.8,6.9,7.0,7.1,7.2,7.3,7.4,7.5,7.6的培养液中培养MRC-5细胞,通过显微技术观察细胞形态、对细胞进行计数,然后对MRC-5细胞的生长情况进行分析比较。结果在不同pH值的培养液中,MRC-5细胞的生长状况不同,细胞数量和细胞形态都有差异,当培养液的pH值为7.2-7.3时,细胞生长形态最好,细胞数量最高可达2.37×106/ml。结论研究表明,培养液的pH值为7.2-7.3时,MRC-5细胞的生长状况最佳。Objective To screen the optimized pH for culturing the human embryonic lung diploid fibroblasts MRC-5 cells. Methods MRC-5 cells were cultured in the medium with different pH（6. 8,6. 9,7. 0,7. 1,7. 2,7. 3,7. 4,7. 5,7. 6）. The morphology was observed under the microscope,and the number of cells was counted. The growth of the MRC-5 cells cultured at different pH was compared. Results In the medium of different pH,MRC-5 cell number and morphology were different. When the pH value was 7. 2-7. 3,the MRC-5 cell morphology was the best,and the cell numbers grew up to 2. 37 × 106/ ml. Conclusion The pH of 7. 2- 7. 3 is the optimized value for culturing the MRC-5 cells.

关 键 词：人胚肺二倍体成纤维细胞 MRC-5 PH值 细胞培养 

分 类 号：Q813.1[生物学—生物工程]