血清前S1、前S2抗原及HBeAg联合检测对HBV感染诊断的临床意义  

Clinical significance of combined detection of serum pre-S1,pre-S2 antigen and HBeAg for diagnosis of HBV infection

作　　者：段虹舫 

机构地区：[1]广州市番禺区南村医院检验科,广东广州511442

出　　处：《国际检验医学杂志》2014年第9期1112-1113,共2页International Journal of Laboratory Medicine

基　　金：广州市番禺区科技和信息化局课题基金资助项目(2013Z03-55)

摘　　要：目的探讨血清前S1、前S2及乙型肝炎病毒(HBV)e抗原(HBeAg)联合检测对HBV感染诊断的临床意义。方法采用酶联免疫吸附测定(ELISA)对450例慢性乙型病毒性肝炎患者血清前S1、前S2抗原及HBeAg进行检测,采用实时荧光定量聚合酶链反应(FQ-PCR)对其HBV DNA进行检测。结果在不同HBV DNA载量患者中,血清前S1、前S2及HBeAg有不同的表达,载量越高,表达越高(P<0.05);前S1、前S2及HBeAg均与HBV DNA具有相关性(P<0.05),相关性由高到低依次为HBeAg、前S1及前S2抗原。3项联合检测与HBV DNA的相关性最高;3项指标阳性患者的HBV DNA阳性比例明显高于单项及2项阳性患者(P<0.05)。结论血清前S1、前S2及HBeAg联合检测能更好地反映HBV的复制。Objective To investigate the clinical significance of combned detection of serum pre-S1 ,pre-S2 antigen and hepatitis B virus（HBV） e antigen（HBeAg） for diagnosis of HBV infection .Methods Enzyme-linked immunosorbent assay（ELISA） was employed to detect the serum pre-S1 ,pre-S2 antigen and HBeAg of 450 patients with chronic hepatitis B and real-time fluorescent quantitative polymerase chain reaction （FQ-PCR） was used to detect their HBV DNA .Results In patients with different HBV DNA levels ,serum pre-S1 ,pre-S2 antigen and HBeAg showed different expression ,the higher the load ,the higher the expression （P〈0 .05） .Pre-S1 ,pre-S2 antigen and HBeAg were all correlated with HBV DNA （P〈0 .05） and the descending order of rele-vance were HBeAg ,pre-S1 and pre-S2 antigen .Combied detection of the three indicators showed the highest correlation with HBV DNA .The positive proportion of HBV DNA of patients with the three indicators positive was markedly higher than those in pa-tients with single or two indicators positive （P〈0 .05） .Conclusion Combind detection of serum pre-S1 ,pre-S2 antigen and HBeAg may reflect well on HBV replication .

关 键 词：肝炎病毒 乙型 抗原 前S1 抗原 前S2 肝炎e抗原 乙型 

分 类 号：R512.62[医药卫生—内科学]