不同的检验方法对沙眼衣原体检测结果的影响与评价  

Effects and evaluation of different test methods on the results of Chlamydia trachomatis detection

作　　者：黄江浩[1] 陈宝娜[1] 常帅[1] 刘平[1] 叶佐婉[1] 於艳霞[1] 杨庆伟[1] 

机构地区：[1]广州医科大学附属深圳沙井医院检验科,广东深圳518104

出　　处：《国际检验医学杂志》2014年第9期1168-1168,1171,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨并评价不同的检验方法对沙眼衣原体检测结果的影响。方法采用酶联免疫吸附测定(ELISA)法与免疫胶体金技术对354例标本进行沙眼衣原体检测,并对两种方法的检测结果进行比较。结果 ELISA与免疫胶体金技术检测结果比较,总体标本及女性标本检测结果的差异无统计学意义(P>0.05),ELISA检测男性标本的阳性检出率为11.02%(13/118),显著高于女性标本[4.2%(5/118)](P<0.05)。结论 ELISA法特异性、敏感性高于免疫胶体金技术,对男性尿道沙眼衣原体感染的早期诊断具有重要意义。Objective To investigate and evaluate the effects of different test methods on the results of Chlamydia trachomatis detection .Methods Enzyme-linked immunosorbent assay（ELISA） method and the immune colloidal gold technique were adopted to detect the Chlamydia trachomatis in 354 specimens .Results Compared the detection results of ELISA and immune colloidal gold technique ,differences of detection rates of overall specimens and female specimens was not statistically significant （P〉0 .05） .The positive rate of male specimens detected by ELISA was 11 .02% （13/118） ,which was significantly higher than that of female speci-mens[4 .2% （5/118）]（P〈0 .05） .Conclusion The specificity and sensitivity of ELISA were higher than those of immune colloidal gold technique ,which is important for the early diagnosis of male urethral Chlamydia trachomatis infection .

关 键 词：衣原体 沙眼 酶联免疫吸附测定 免疫胶体金技术 

分 类 号：R446.6[医药卫生—诊断学]