生肌因子Myogenin和Myf5真核双表达载体的构建及效果检测  

Construction and Effect Test of the Eukaryotic Co-expression Vector for Myogenin and Myf5

作　　者：杜朝[1] 孙洪敏[1] 王英泽[1] 孙国杰[1] 

机构地区：[1]河北科技大学生物科学与工程学院,河北石家庄050018

出　　处：《华北农学报》2014年第2期13-17,共5页Acta Agriculturae Boreali-Sinica

基　　金：河北科技大学博士科研启动基金项目(自然科学QD200962);国家自然基金青年科学基金项目(31100715);国家自然科学基金面上项目(81171455)

摘　　要：为研究生肌因子如Myogenin和Myf5在调控肌特异基因表达时的作用机制,将这2个因子共转染非肌细胞,为确保转染效果,依次将这2个因子的编码序列克隆在双表达载体pVITRO2上。测序结果显示,克隆到载体的myogenin和Myf5编码区序列正确;初步的功能检测表明,它们都能有效地激活各自的靶基因。Muscle development was specially controlled by the myogenic factors such as myogenin and Myf5. It is necessary to cotransfect these two myogenic factors into non-muscle cells for further investigating the machanism by which they regulate the muscle specific genes. To gain high transfection efficiency,the coding sequences of myogenin and Myf5 were cloned successively into the pVITRO2,an expression vector containing two multiple cloning sites（ MCS） for the convenient cloning of two cDNAs. Sequencing results proved that the cloned myogenin and Myf5 are correct,and the transfection assay suggested they could activate their respective target genes respectively.

关 键 词：生肌过程 双表达载体 生肌因子 

分 类 号：Q31[生物学—遗传学]