灰葡萄孢菌核发育相关基因的功能分析  

Functional Analysis of a Gene Related to Sclerotia Formation in Botrytis cinerea

作　　者：司贺龙[1] 赵斌[1] 赵福鑫[1] 邢继红[1] 韩建民[1] 董金皋[1] 

机构地区：[1]河北农业大学真菌毒素与植物分子病理学实验室,河北保定071001

出　　处：《华北农学报》2014年第2期80-84,共5页Acta Agriculturae Boreali-Sinica

基　　金：河北省科技支撑计划项目(NO.12226507);河北农业大学青年科学基金项目(No.QJ201235;QJ201231)

摘　　要：从灰葡萄孢ATMT突变体库中筛选获得一株不产生菌核的突变体,并利用TAIL-PCR技术获得插入位点的侧翼序列,将其在灰葡萄孢基因组数据库中进行Blast比对分析,采用分子生物学技术,对突变菌株BMH174鉴定并获得其突变基因(BC1G_06945.1);并对突变体的形态学、生长速率、分生孢子产量、致病能力、胞壁降解酶活力、毒素的生物学活性等方面进行了研究,这将为深入了解灰葡萄孢的致病机制奠定基础。One sclerotia-defect mutant of Botrytis cinerea BMH174 was obtained by Agrobacterium tumefaciensmediated method. The flanking sequence was acquired by TAIL-PCR, and then the T-DNA insertion gene confirmed by Blast in the B. cinerea gene database. The mutant gene was identified BC1G_06945.1 gene by molecular biology. The sclerotia formation and pathogenicity in B. cinerea which will facilitate to understand molecular mechanism of conidium development, sclerotia formation and pathogenic in B. cinerea.

关 键 词：灰葡萄孢 T-DNA插入突变体 TAIL-PCR 

分 类 号：S436.631[农业科学—农业昆虫与害虫防治]