大鼠广泛肠切除后小肠组织miR-125a和Mcl-1的表达及意义  

Expression and significance of miR-125a and Mcl-1 in intestinal tissue after massive small bowel ;resection in rat

作　　者：咸孟飞 马晋平[1] 陈斯乐[1] 林建伟[1] 何伟玲[1] 杨东杰[1] 吴晖[1] 陈创奇[1] 蔡世荣[1] 

机构地区：[1]中山大学附属第一医院胃肠胰腺外科,广州510080

出　　处：《中华胃肠外科杂志》2014年第5期495-498,共4页Chinese Journal of Gastrointestinal Surgery

基　　金：广东省科技计划项目（2011B031800271）;大亚湾科技计划项目（2013A01015）

摘　　要：目的：探讨广泛肠切除后miR-125a和抗凋亡蛋白（Mcl-1）在肠上皮细胞中表达的变化及其在短肠综合征剩余肠管适应性变化过程中的意义。方法选取8周龄的SD雄性大鼠54只，分为实验组（18只，进行70％的大范围小肠切除，距回盲部近端15 cm和Treitz韧带远端10 cm处分别切断小肠，然后行空回肠端端吻合）、肠切除对照组（18只，在距回盲部近端15 cm处切断肠管然后再吻合）和手术对照组（18只，仅行开关腹手术）。术后1周在距吻合口远端1 cm处取材，采用免疫组织化学方法和实时荧光定量 PCR检测各组大鼠小肠组织中 Mcl-1和 miR-125a 表达。结果实验组大鼠肠组织中Mcl-1阳性表达率为18．8％（3／16），明显低于手术对照组（76．5％，13／17）和肠切除对照组（83．3％，15／18）（均P＜0．01）；miR-125a相对表达量为1.92，明显高于肠切除对照组（1.01）和手术对照组（1.05）（均P＜0．01）。结论 miR-125a和Mcl-1在广泛肠切除诱导的肠道适应过程中发挥重要作用，二者之间通过一定的机制相互调节。Objective To investigate the expression and significance of miR-125a and anti-apoptotic protein Mcl-1 in intestinal tissue after massive small bowel resection in intestinal adaptation. Methods Sprague-Dawley rats （54 male rats, 8-week old） were divided into 3 groups randomly, including two control groups. Rats in the experiment group were subjected to 70% massive small bowel resection. Rats in the resection group underwent simple intestinal resection and anastomosis. Rats in the control group underwent laparotomy alone. A 5 cm intestine approximately 1 cm distal to the anastomosis was harvested a week after operation. Expression of Mcl-1 was assessed by immunohistochemistry and real-time PCR was used to detect the expression of miR-125a in intestinal tissue. Results The positive expression of Mcl-1 in the experiment group was 18.8%（3/16）, significantly lower than that in the control group（76.5%, 13/17） and the resection group （83.33%, 15/18）（both P〈0.01）. The expression of miR-125a in the experiment group was 1.92, significantly higher than that in the control group（1.01） and the resection group （1.05）（both P〈0.01）. Conclusion miR-125a and anti-apoptotic protein Mcl-1 may play an important role in intestinal adaptation process and they may regulate each other through a certain pathway.

关 键 词：肠切除术 短肠综合征 MCL-1 MCL-1 

分 类 号：R656[医药卫生—外科学]