2006—2011年青岛地区B型流感病毒全基因组进化特征分析  

Whole-genome evolution of influenza B viruses prevalent in Qingdao from 2006 to 2011

作　　者：杨婷婷[1] 柴青[2] 段海平[2] 于萍[2] 刘晓琳[2] 汪照国[2] 

机构地区：[1]青岛大学附属医院检验科,266003 [2]山东省青岛市疾病预防控制中心,266033

出　　处：《中华微生物学和免疫学杂志》2014年第4期269-277,共9页Chinese Journal of Microbiology and Immunology

基　　金：青岛市公共领域科技支撑计划项目（10-3-3-8-1-nsh）

摘　　要：目的：了解2006-2011年间青岛地区流行的B型流感病毒的全基因组进化特征。方法选取2006-2011年间青岛地区分离的B型流感病毒，提取病毒RNA，应用逆转录聚合酶链反应（ RT-PCR）扩增各基因片段，并进行序列测定；Sequencher 软件完成序列拼接。 MEGA5.0软件对各基因片段进行系统发育分析及分子特征分析。结果在血凝素（ HA）基因进化树上，Victoria系V1分支为B/Malaysia/2506/2004样毒株，包含2006-2009年13个毒株，V2分支为B/Brisbane/60/2008样毒株，包含2009-2011年12个毒株；Yamagata 系全部为 B/Florida/4/2006样毒株， Y1分支包含2006-2008年5个毒株，Y2分支包含2010-2011年7个毒株。全基因组分析表明V2分支3个毒株发生了5＋3重配，1个毒株发生了1＋7重配，重配株均与前一季及当季疫苗株同亚型；HA进化树上处于Yamagata系的毒株全部属于基因型2，处于Victoria系的毒株全部属于基因型15；HA1蛋白与疫苗株进行比较：Victoria系分离株主要变异位点包括H14Q、L58P、N129S、I146V、N171D、R279K；Yamaga-ta系分离株主要变异位点包括R48K、K88R、P108A、N116K、S150I、N165Y、D196N、N202S、S229G；位点116和129、位点150、位点165、位点196和202分处以往证实经历阳性选择的抗原表位120环、150环、160环和190环。结论2006-2011年青岛地区B型流感病毒两系共流行，并且持续进化；疫苗的使用可为同系病毒造成选择压力，而双组分的B型流感疫苗可能更为有效。Objective To study the whole-genome evolution of influenza B viruses prevalent in Qingdao from 2006 to 2011 .Methods RNA was extracted from influenza B viruses isolated in Qingdao from 2006 to 2011 .Each gene segment was amplified by reverse transcription polymerase chain reaction （ RT-PCR） and then sequenced .Gene sequences of each virus were determined and assembled by using Sequench -er software .A phylogenetic analysis for each gene segment was conducted by using MEGA 5.0 software pack-age.Results The phylogenetic tree of hemagglutinin （ HA） gene showed that 13 strains from 2006 to 2009 belonging to V1 clade of Victoria lineage were B/Malaysia/2506/2004-like viruses,and 12 strains from 2009 to 2011 belonging to the V2 clade of Victoria lineage were B/Brisbane/60/2008-like viruses.Moreover, strains of Yamagata lineage were all B/Florida/4/2006-like viruses including 5 strains of Y1 clade circulated from 2006 to 2008 and 7 strains of Y2 clade circulated from 2010 to 2011, respectively.The analysis of whole-genome evolution showed that 3 viruses of V2 clade presented 5＋3 reassortment and 1 virus presented 1＋7 reassortment.All reassortant strains matched with the vaccine strains of the present and previous season . The Yamagata and Victoria lineage strains belonged to genotype 2 and genotype 15,respectively.Compared with vaccine strains , the HA1 protein of Victoria lineage strains showed mutations at amino acid sites of H14Q, L58P, N129S, I146V, N171D and R279K, while R48K, K88R, P108A, N116K, S150I, N165Y, D196N,N202S and S229G amino acid mutations were mainly detected in Yamagata lineage strains .The sites 116 and 129,150,165,196 and 202 located in the 120,150,160 and 190 loops,respectively,which had been＆amp;nbsp;previously determined to be the hotspots under positive selection .Conclusion Both Yamagata and Victoria lineages of influenza B viruses were prevalent in Qingdao and evolved continuously from 2006 to 2011 .The selective pressure that a vaccine would provide was only to virus strains be

关 键 词：流感病毒B型 全基因组 抗原变异 进化 

分 类 号：R373.13[医药卫生—病原生物学]