肺炎链球菌荚膜多糖抗原活性的评价方法  

A comparative study of a double immune-diffusion assay and a rate nephelometer analysis for evalua-ting the antigen activity of pneumococcal capsular polysaccharides

作　　者：石继春[1] 沈荣[2] 刘韵乐[2] 陈琼[1] 王春娥[1] 谢贵林 叶强[1] 

机构地区：[1]中国食品药品检定研究院卫生部生物技术产品检定方法及其标准化重点实验室,北京100050 [2]兰州生物制品研究所有限责任公司 [3]中国生物技术股份有限公司,北京100029

出　　处：《中华微生物学和免疫学杂志》2014年第4期304-309,共6页Chinese Journal of Microbiology and Immunology

基　　金：国家863计划（2012AA02A402）

摘　　要：目的比较免疫双扩散法和速率散射比浊分析法在肺炎链球菌荚膜多糖抗原性评价方面的优劣。方法分别采用免疫双扩散法和速率散射比浊分析法检定来源于4个公司和美国ATCC的1型、6B型、9V型、10A型、14型和19A型肺炎链球菌荚膜多糖样品的抗原活性，同时比较肺炎链球菌免疫血清和仪器增益值对实验结果的影响。结果两种抗原活性检定方法都显示9V型4号样品的抗原活性缺失，其速率散射浊度信号值接近阴性对照；其余多糖样品均有沉淀线，速率散射浊度信号值高低不等；不同来源的肺炎链球菌抗血清和仪器增益值对速率散射比浊分析法的实验结果无明显干扰。结论速率散射比浊分析法能够定量分析各型肺炎链球菌荚膜多糖的抗原活性。Objective To comparatively analyze the advantages and disadvantages between a double immune-diffusion assay and a rate nephelometer analysis for the detection of antigen activity of pneu- mococcal capsular polysaccharides. Methods The antigen activity of pneumococcal capsular polysaccharides of serotypes 1,6B,9V, 10A, 14 and 19A from four manufacturers and ATCC were analyzed by a double im- mune-diffusion assay and a rate nephelometer analysis, respectively. The effects of antiserum samples and gain values on the rate response value were evaluated. Results The sample 4 of type 9V showed no antigeni- city with a rate response value similar to that of negative control as indicated by both tests. However,the pre- cipitation lines and the rate response values presented by other polysaccharide samples differed in a wide range. Results of the rate nephelometer analysis were not affected by the anti-serum samples from different sources and the gain values. Conclusion The rate nephelometer analysis could quantitatively analyze the antigen activity of pneumococcal capsular polysaccharides.

关 键 词：肺炎链球菌 荚膜多糖 速率散射比浊分析法 抗原活性 

分 类 号：R446.6[医药卫生—诊断学]