低强度周期性应力对骨骼肌细胞增殖分化调控的机制  

The mechanism of low cyclic stretch in cell proliferation and differentiation

作　　者：匡威[1] 谭家莉[2,3] 王桥[1] 李洪涛[1] 伍丽静 刘琴瑶[1] 

机构地区：[1]广州军区广州总医院口腔科,广东广州510010 [2]中山大学光华口腔医学院附属口腔医院,广东广州510055 [3]广东省口腔医学重点实验室,广东广州510055

出　　处：《临床口腔医学杂志》2014年第5期259-262,共4页Journal of Clinical Stomatology

基　　金：广东省自然科学基金(52012010009495);广东省科技计划中山大学(2012B031800185);中山大学"985"工程及中山大学教师培养计划(BYKPY42)

摘　　要：目的:探明低强度周期性应力调控骨骼肌细胞增殖分化的分子机制。方法:采用Flexercell Strain Unit系统对C2C12成肌细胞加载5%牵张应力,观察细胞增殖和分化情况,检测成肌细胞分化标志基因myogenin等的表达情况,荧光报告系统检测NFkappa B的活性,microRNA特异性荧光定量PCR检测miR146a在增殖和分化条件下表达差异。结果:在5%周期性牵张应力作用下,成肌细胞分化受到抑制;无论增殖条件还是分化条件下,低强度拉伸均可以导致miR146a升高,但在分化条件下更为明显(P<0.05);分化条件下组成性的NFkappa B的活性低于其在增殖条件下的活性(P<0.05)。结论:低强度应力抑制骨骼肌细胞分化,可能是通过miR146a的升高从而抑制NFkappaB的活性引起的。Objective： To investigate the regulation and the molecular mechanism of low cyclic stretch in proliferation and differentiation of skeletal muscle cells, nethod：Flexercell Strain Unit system was used to load cyclic stretch to myoblasts C2C12. 5 % cyclic stretch was loaded to observe the proliferation and differentiation of myoblasts. The expression of differentiation marker genes such as myogenin were tested. Fluorescent reporting system was induced to detect the NFkappaB activity and miRNA specific fluorogenic quantitative PCR was induced to test the difference of the miR146a expression under proliferation and differentiation culture condition. Result： When myoblasts were under 5 % cyclic stretch, the differentiation is inhibited. The expression of miR146a increases both in proliferation and differentiation culture condition under low cyclic stretch and it＇s much obvious in differentiation culture condition （P 〈0.05）. The NFkappaB activity in differentiation culture condition is lower than that in proliferation group （P 〈0.05）. Conclusion： Low cyclic stretch can inhibit the differentiation of skeletal muscle cell, which may due to the inhibition of the NFkappaB activity with increased expression of miR146a.

关 键 词：周期性应力 骨骼肌细胞 增殖 分化 miR146a 

分 类 号：Q813.1[生物学—生物工程]