猪骨羟基磷灰石的体外溶解行为研究  

In vitro solubility behavior of porcine hydroxyapatite

作　　者：张汉卿[1,2] 韩艾芳[1,2] 李志鹏[1,2] 黄宝鑫[1,2] 陈卓凡[1,2] 

机构地区：[1]中山大学光华口腔医学院.附属口腔医院种植科,广东广州510055 [2]广东省口腔医学重点实验室,广东广州510080

出　　处：《临床口腔医学杂志》2014年第5期266-268,共3页Journal of Clinical Stomatology

基　　金：国家自然科学基金面上项目(30973355);广东省自然科学基金(S2011010004978)

摘　　要：目的：探讨猪骨羟基磷灰石（PHA）的体外溶解行为.方法：运用固体滴定-激光散射监测法测定PHA在0.1 mol·L^-1 KCl溶液中的37℃等温溶解线;以钙离子活度计监测PHA浸泡于超纯水中的Ca^2＋释放动态变化.结果：PHA在pH值为3.0～5.0的0.1 mol·L^-1 KCl溶液中的溶解度与化学合成的羟基磷灰石（HA）差异无统计学意义,但显著低于牛骨羟基磷灰石,溶解度随pH值降低而升高;超纯水浸泡液中Ca^2＋浓度恒定维持在30～35×10^-6范围内,不受浸泡时间和浸泡物质量影响,更新浸泡液后PHA继续释放Ca^2＋至原始浓度.结论：PHA的溶解度与溶液pH值密切相关,在中性溶液中可释放Ca^2＋且浓度稳定.Objective：This study was to explore the in vitro solubility behavior of porcine hydroxyapatite （PHA）.Method：The solubility isotherm of PHA in 0.1 mol·L^-1 aqueous KCl at 37 ℃ was determined with solid titration and laserscattering end-point detection approach.Ion activity meter was used to measure the continuous variation of the free calcium releasing from PHA in ultrapure water.Result：No significant difference was found between PHA and synthetic hydroxyapatite on the solubility in 0.1 mol·L^-1 aqueous KCl,ranging from the pH of 3.0～5.0.The solubility increased significantly with the decrease of pH value.The Ca^2＋ concentration in neutral soaking solution remained in the range of 30～35 ppm and was unaffected by soaking time and PHA amount.Ca^2＋ was released from PHA consequently after solution refreshed until the concentration above.Conclusion：Solubility of PHA was highly related to the solution pH.The concentration of Ca^2＋ released from PHA was maintained a constant in neutral solution.

关 键 词：猪骨羟基磷灰石 溶解度 固体滴定 体外溶解 

分 类 号：R318.08[医药卫生—生物医学工程]