益精方对不育症大鼠睾丸生精细胞凋亡及Bcl-2、Bax蛋白表达的影响  

Effect of Yijing Recipe on Apoptosis of Testis Spermatogenic Cells and the Expression of Bcl-2 and Bax Protein in Infertility Rats

作　　者：王力[1] 陈东[2] 闵泽[1] 王家辉[2] 

机构地区：[1]辽宁中医药大学第一临床学院,沈阳110847 [2]海南医学院中医学院,海口571199

出　　处：《中国中西医结合杂志》2014年第5期602-605,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81260591);海南省自然科学基金资助项目(No.310050)

摘　　要：目的观察益精方对腺嘌呤法不育症大鼠睾丸生精细胞凋亡及Bcl-2/Bax蛋白表达的影响。方法 Wistar大鼠75只随机分为空白组,模型组,益精方高、中、低剂量组,每组15只。除空白组外,其余各组均以腺嘌呤连续灌胃10天;从第11天开始,空白组及模型组用等量生理盐水灌胃,益精方高(3.38g/100g)、中(1.69g/100g)、低剂量(0.85g/100g)组分别以益精方各剂量灌胃,每天1次,连续20天。实验结束后将大鼠全部处死,摘取睾丸,采用原位缺口末端标记法(TUNEL)和免疫组化SABC法分别检测各组动物生精细胞的凋亡情况及Bcl-2/Bax蛋白的表达。结果与空白组比较,模型组Bcl-2蛋白表达降低,Bax蛋白表达升高,细胞凋亡数升高,差异均有统计学意义(P<0.01)。与模型组比较,三个剂量组Bcl-2蛋白表达均升高(P<0.01,P<0.05),高、中剂量组Bax蛋白表达均降低(P<0.01,P<0.05),中剂量组细胞凋亡数降低(P<0.01)。结论益精方能够通过调控睾丸组织Bcl-2及Bax蛋白的表达,抑制生精细胞的凋亡。Objective To observe the effect of Yijing Recipe （YR） on the apoptosis of testis spermatogenic cells and the protein expression of Bcl-2/Bax in rats with adenine induced infertility. Methods Totally 75 Wistar rats were randomly divided into 5 groups, i.e., the blank control group, the model group, the high dose YR group, the middle dose YR group, and the low dose YR group, 15 in each group. Except those in the blank control group, rats in the rest groups were intragastrically administered with adenine for 10 successive days. From the 11th day, rats in the blank control group and the model group were fed with equal volume of normal saline. Rats in the YR groups were intragastrically administered with YR at different doses （3.38 g/100 g; 1.69 g/100 g; 0.85 g/100 g）, once daily for 20 consecutive days. All rats were killed by the end of the experiment and their testes extracted. The apoptosis of spermatogenic cells and the expression of Bcl-2/Bax proteins were detected by terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling （TUNEL） and SABC method. Results Compared with the blank control group, the Bcl-2 protein expression decreased, the Bax protein expression increased, and the apoptosis index increased in the model group, showing statistical difference （P 〈0.01 ）. Compared with the model group, the Bcl-2 protein expression increased in the three YR treated groups （P 〈0.01, P 〈0.05）. The Bax protein expression level decreased in the high and middle dose YR groups （P 〈0.01, P 〈0.05）. The apoptosis index decreased in the middle dose YR group （P 〈0.01 ）. Conclusion YR could inhibit the apoptosis of spermatogenic cells through regulating the expression of Bcl-2 and Bax protein in the testis.

关 键 词：益精方 腺嘌呤 BCL-2蛋白 BAX蛋白 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]