白藜芦醇对高糖诱导内皮细胞损伤代谢记忆效应的研究  

Effects of resveratroi on high glucose memory-induced injury to endothelial cell

作　　者：丁慧[1] 王鹏[1] 王颜刚[1] 

机构地区：[1]青岛大学医学院附属医院内分泌科,266003

出　　处：《中华内分泌代谢杂志》2014年第5期432-434,共3页Chinese Journal of Endocrinology and Metabolism

摘　　要：人脐静脉内皮细胞分为正常对照组、甘露醇对照组、高糖组和白藜芦醇低、中、高剂量组，细胞于30mmol／L葡萄糖和0．1、1和10μmoL／L白藜芦醇培养16h后．再以5．5mmol／L葡萄糖培养6d，应用ELISA法检测细胞培养上清中血管细胞黏附分子1（VCAM-1）、单核细胞趋化蛋白1（MCP-1）和纤溶酶原激活物抑制剂1（PAI-1）水平，以Western印迹法检测细胞内NF-κB蛋白表达。与正常对照组相比，高糖可诱导内皮细胞NF-κB表达上调（P〈0．05），同时VCAM-1、MCP-1和PAI-1水平升高（均P〈0．05），且在糖浓度恢复正常后仍持续上升。与高糖组相比，白藜芦醇预处理可以明显减少NF-κB表达．降低VCAM-1、MCP-1和PAI-1水平（均P〈0．05）。结果提示白藜芦醇可能通过NF—κB通路降低VCAM-1、MCP—1和PAI-1水平，改善高糖代澍记忆效应介导的内皮细胞损伤。Cultured human umbilical vein endothelial cells （HUVECs） were divided and assigned to 6 groups： normal control group, mannitol control group, high glucose group, low dose resveratrol group, medium dose resveratrol group, and high dose resveratrol group. HUVECs were pretreated with or without 30 mmol/L glucose plus various concentrations of resveratrol （0.1,1,10 μmol/L） for 16 hours and then incubated with 5.5 mmol/L glucose for 6 days. The levels of vascular cell adhesion molecule 1 （ VCAM-1 ）, monocyte ehemotaetic protein 1 （ MCP-1 ）, and plasminogen activator inhibitor 1 （ PAI-1 ） in the euhure supematant were measured by ELISA. NF-κB expression was detected by Western blot. Compared with normal glucose group, high glucose up-regulated the expression of NF-κB, along with the increased levels of VCAM-1, MCP-1, and PAI-1 （ all P〈0.05 ） , which were still maintained at high levels even after withdrawal of high glucose. Resveratrol treatment down-regulated the expression of NF-κB and lowered the levels of VCAM-1, MCP-1, and PAI-1 （ all P〈0.05 ）. These results suggest that resveratrol may decrease the secretion of VCAM-1, MCP-1, and PAI-1 and prevent high glucose memory-induced injury to endothelial cell via NF-κB pathway.

关 键 词：白藜芦醇 人脐静脉内皮细胞 代谢记忆 核因子ΚB 

分 类 号：R587.1[医药卫生—内分泌]