动脉粥样硬化大鼠动脉的基因芯片分析  

Gene Chip Analysis for Artery of Rats with Atherosclerosis

作　　者：钟武[1] 荣华[1] 陈睦虎[1] 余平贵[1] 王亮[1] 何延政[1] 

机构地区：[1]泸州医学院附属医院急诊科,四川泸州646000

出　　处：《华西医学》2014年第5期867-870,共4页West China Medical Journal

摘　　要：目的用基因芯片技术检测正常Wistar大鼠和动脉粥样硬化Wistar大鼠腹主动脉组织中差异表达的基因,以探讨动脉粥样硬化的发病机制。方法正常对照组大鼠给予正常饮食及生理盐水腹腔注射;动脉粥样硬化模型大鼠给予高脂饮食及维生素D负荷,并以球囊损伤动脉。90 d后测定血浆总胆固醇、甘油三酯水平以确定动脉粥样硬化模型建立成功,取正常大鼠和动脉粥样硬化大鼠的腹主动脉进行切片及苏木精-伊红染色观察病理学差异,提取腹主动脉组织mRNA做基因芯片检查。结果与正常对照组相比,动脉粥样硬化组大鼠血浆内总胆固醇、甘油三脂水平增高(P<0.01);组织切片证实动脉粥样硬化形成;基因芯片检测结果显示511种基因表达上调,1 219种基因表达下调,其中部分基因与脂质代谢、炎症反应、氧化应激、细胞凋亡相关。结论动脉粥样硬化时血管组织多种基因发生表达水平的改变,可能存在复杂的网络调控。对其中重点基因进行深入研究有助于预防动脉粥样硬化发生和改善该病的预后。Objective To investigate the pathogenesis of atherosclerosis （AS） by detecting different expression genes between normal Wistar rats and rats with atherosclerosis through the technology of gene chip. Methods The rats were treated with standard diet with saline injection （control group） or high-cholesterol diet with vitamin D3 injection and balloon injury （model group）. Total cholesterol （TC） and triglyeride （TG） in serum were detected 90 days later to ensure the success of establishment of the atherosclerosis model. Abdominal aorta was isolated and stained with HE. Total RNA was isolated from the aorta for gene chip analysis to explore the differential gene expression. Results Compared with the control group, the TC and TG level in the model group were highly advanced （P 〈 0.05）. AS model was confirmed by pathological observation. Gene chip detection showed that 511 genes were up-regulated and 1 219 ones were down-regulated which were interrelated with lipid metabolism, inflammatory reaction, oxidative stress and apoptosis as well. Conclusions The expression change with multiple gene in AS suggests that the nosogenesis of AS is adjusted and controlled complicatedly. Intensive study of some important genes will contribute to the prevention and improvement of prognosis of AS.

关 键 词：基因芯片 动脉粥样硬化 大鼠 

分 类 号：R440[医药卫生—诊断学]