海洋真菌杂色曲霉F62丁内酯类化合物研究  

Butyrolactone derivatives isolated from the marine fungus Aspergillus versicolor F62

作　　者：巩婷[1] 董世豪[1] 朱平[1] 

机构地区：[1]天然药物活性物质与功能国家重点实验室国家卫生和计划生育委员会天然药物生物合成重点实验室中国医学科学院北京协和医学院药物研究所,北京100050

出　　处：《菌物学报》2014年第3期706-712,共7页Mycosystema

基　　金：国家"十二五"科技重大专项"综合性新药研究开发技术大平台"(No.2012ZX09301002-001-005);中央高校基本科研业务费(No.2012N06)

摘　　要：为了深入研究相似蜂海绵相关真菌杂色曲霉F62的活性代谢产物,采用硅胶柱、凝胶柱色谱和HPLC等分离手段对其大米固体发酵物的乙酸乙酯提取物进行分离,并利用质谱和核磁共振等现代波谱学技术对其结构进行鉴定,从中共得到6个丁内酯类化合物,分别为丁内酯Ⅰ(1)、丁内酯Ⅱ(2)、丁内酯Ⅲ(3)、丁内酯Ⅳ(4)、丁内酯Ⅶ(5)、Aspernolides A(6),其中化合物1在10μmol/L时表现出较强的抗炎活性,其IC50值为8.73μmol/L。Chemical diversity investigation for searching novel bioactive compounds from secondary metabolites of the marine fungus Aspergillus versicolor F62 was carried out. Various modern separation methods and spectral techniques were employed for isolation and identification of target compounds. Their structures were elucidated by detailed spectroscopic analysis and comparison with literature data. Six butyrolactone derivatives were identified as butyrolactone I （1）, butyrolactone II （Z）, butyrolactone III （3）, butyrolactone IV （4）, butyrolactone VII （5）, and Aspernolide A （6）. Compound 1 exhibited moderate anti-inflammatory activity at the concentration of lOl^mol/L and the Cso value was 8.73μmol/L.

关 键 词：相似蜂海绵 杂色曲霉 次生代谢产物 丁内酯 

分 类 号：Q946[生物学—植物学]