芫花花蕾中甾醇类化合物的分离与鉴定  

Isolation and identification of the sterols from the flower buds of Daphne genkwa Sieb. et Zucc.

作　　者：羡冀[1] 徐扬[2] 罗显峰[1] 于韬[1] 孙倩[3] 李玲芝[3] 

机构地区：[1]辽宁省肿瘤医院药学部,辽宁沈阳110042 [2]海南碧凯药业有限公司,海南海口570216 [3]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2014年第5期347-350,共4页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金资助项目(81273388);沈阳市科技计划资助项目(F11-265-6-01)

摘　　要：目的对瑞香科瑞香属植物芫花（Daphne genkwa Sieb.et Zucc.）花蕾中的甾醇类化学成分进行分离与结构鉴定。方法运用硅胶﹑Sephadex LH-20柱色谱﹑重结晶等分离手段进行甾醇类化合物的分离纯化,根据理化性质及波谱数据鉴定其化学结构。结果从芫花花蕾的体积分数95%乙醇提取物中分离得到6个化合物,分别鉴定为：豆甾-5-烯-3β,7α-二醇（stigmasta-5-ene-3β,7α-diol,1）、豆甾-5-烯-3β,7β-二醇（stigmasta-5-ene-3β,7β-diol,2）、豆甾-4-烯-3β,6β-二醇（stigmasta-4-ene-3β,6β-diol,3）、7-ketositosterol（3β-hydroxysitost-5-ene-7-one,4）、过氧化麦角固醇（ergosterol peroxide,5）和β谷甾醇-3β-吡喃葡萄糖苷-6＇-O-棕榈酸酯（β-sitosteryl-3β-glucopyranoside-6＇-O-palmitate,6）。结论化合物2~6为首次从瑞香属植物中分离得到,化合物1为首次从芫花植物中分离得到。Objective To study the chemical constituents of Daphne genkwa Sieb. et Zucc. Methods The compounds w ere isolated by chromatography on silica gel,Sephadex LH-20 and recrystallization,and their structures w ere identified on the basis of spectrascopic analysis and physico-chemical constants. Results Six compounds w ere isolated from 95% ethanol extract from buds of Daphne genkwa and identified as stigmasta-5-ene-3β,7α-diol（ 1）,stigmasta-5-ene-3β,7β-diol（ 2）,stigmasta-4-ene-3β,6β-diol（ 3）,3β-hydroxysitost-5-ene-7-one（ 4）,ergosterol peroxide（ 5）,β-sitosteryl-3β-glucopyranoside-6＇-O-palmitate（ 6）. Conclusions Compounds 2-6 are firstly isolated from Daphne genus. Compound 1 is isolated from this plant for the first time.

关 键 词：芫花 甾醇 化学成分 结构鉴定 

分 类 号：R284.1[医药卫生—中药学] R914[医药卫生—中医学]