小直径DRG神经元上表达的电压门控离子通道特性  

Character of voltage-activated channels in small DRG neurons

作　　者：刘息芳 陈健钊[1] 杜京楠 李桂霞[1] 李春莉[1] 吴春福[1] 

机构地区：[1]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2014年第5期384-390,共7页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金资助项目(81073081);沈阳市科技计划基金资助项目(F12-277-1-07)

摘　　要：目的建立一种简单易行,满足多种药理学实验需要的大鼠DRG神经元急性分离方法,探讨大鼠DRG神经元的电生理特性。方法对160-220 g SD大鼠DRG神经元进行急性分离,并采用全细胞膜片钳技术记录电压门控离子通道钠电流、钙电流和钾电流。结果急性分离的大鼠DRG神经元呈圆形或椭圆形,胞质均一,折光性好,所表达的钠通道电流、钙电流和钾电流符合其电生理特性。结论运用该方法急性分离细胞操作简单易行,容易获得和辨认,电生理特征明确,适用于疼痛等疾病的电生理研究和相关药物的作用机制考察。Objective To introduce a simple acutely isolated technique,which can meet various pharmacological experiment demands,and study the electrophysiological properties of rat DRG neurons. Methods Acutely isolated DRG neurons from 160-220 g rats and w hole cell patch clamp technique w ere used to record voltage-gated sodium channels,voltage-gated calcium channels,voltage-gated potassium channels. Results DRG neurons w ere round or oval w ith good cytoplasm uniformity and refraction. Their expression of sodium channels,calcium channels,potassium channels w ere agreed w ith their electrophysiological characteristics. Conclusions The methods are simple and easy to obtain and identify DRG neurons. Electrophysiological properties of DRG neurons are typical and clear. These techniques are ideal methods w hich can be applied for the research on electrophysiological properties and the mechanism of medical treatments for diseases in pain and other diseases.

关 键 词：DRG神经元 急性分离方法 膜片钳技术 电生理特征 

分 类 号：R96[医药卫生—药理学]