DM-18型大孔树脂分离纯化新疆沙枣果肉中多糖的工艺  

Study on the Separation and Purification of Elaeagnus Angustifolia Polysaccharide by Using the DM-18 Macroporous Resins

作　　者：杨魁[1] 陈晴晴[2] 杨金凤[2] 杨静[1] 陈景堃[1] 刘红[3] 

机构地区：[1]石河子大学师范学院,石河子832003 [2]石河子大学化学化工学院,石河子832003 [3]石河子大学理学院,石河子832003

出　　处：《石河子大学学报（自然科学版）》2014年第2期232-237,共6页Journal of Shihezi University(Natural Science)

基　　金：教育部国际合作项目"春晖计划"(Z2006-1-83003)

摘　　要：本文研究了DM-18型大孔树脂分离纯化沙枣多糖的工艺条件,考察了各因素对分离、纯化沙枣多糖效果的影响,确定了分离沙枣多糖的最佳分离条件。结果表明:在沙枣多糖样品溶液2.0 mg/mL,上样速率为1.5 BV/h,上样液pH值为7.0,上样量为3.0 BV、洗脱剂乙醇浓度为35%、洗脱剂用量为4.0 BV、洗脱速率为1.0 BV/h时,DM-18型大孔树脂对沙枣多糖的动态吸附率和解吸率分别达到90.13%和92.17%,表明该大孔树脂是一种较好的分离纯化沙枣多糖的材料。To research and optimize the best conditions of the DM-18 macroporous resins,a dynamic absorption and desorption experiment was designed.The result showed that the best conditions as follows：2.0 mg /mL of concentration and 7.0 of pH of the solution of Elaeagnus angustifolia polysaccharide,the sample loading amount of 3.0 BV,1.5 BV/h of the rate,the eluent concentration of 35%,the total quantity of eluent of 4.0 BV and eluent rate of 1.0 BV/h.The rates of absorption and desorption could achieve 90.13% and 92.17% under the conditions above.It concludes that the DM-18 macroporous resins is a good material to separae and purificate the Elaeagnus angustifolia polysaccharide.

关 键 词：沙枣多糖 大孔树脂 分离 纯化 

分 类 号：TQ461[化学工程—制药化工]