麝香保心丸抗心肌细胞缺氧-复氧损伤活性成分筛选  

Screening of main active components against cardiomyocyte hypoxia/reoxygenation injury in shexiang baoxin Pill

作　　者：韩琳[1] 吕超[2] 李敏[2] 黄慧梅[2] 畅婉琳[2] 彭成成[3] 柳润辉[1] 

机构地区：[1]第二军医大学药学院,上海200433 [2]福建中医药大学药学院,福建福州350108 [3]上海交通大学药学院,上海200240

出　　处：《药学实践杂志》2014年第3期209-211,219,共4页Journal of Pharmaceutical Practice

摘　　要：目的：建立原代心肌细胞缺氧-复氧模型，并对麝香保心丸及其20种血中活性成分进行抗缺氧-复氧损伤的活性筛选。方法取新生（1～3 d）SD乳鼠的心脏，建立原代心肌细胞的缺氧-复氧损伤模型，并采用四甲基偶氮唑盐（MTT）比色法进行麝香保心丸及其入血成分的活性筛选。结果麝香保心丸在50μg/ml浓度下具有较好的保护原代心肌细胞缺氧-复氧损伤的作用；人参皂苷Rb1、人参皂苷Rb2、蟾毒灵和麝香酮具有较好的保护原代心肌细胞缺氧-复氧损伤的作用。结论麝香保心丸具有抗心肌细胞缺氧-复氧损伤的作用，人参皂苷Rb1、人参皂苷Rb2、蟾毒灵和麝香酮是其主要的有效成分。该研究为麝香保心丸深入的药效和机制研究奠定了基础。Objective To build hypoxia/reoxygenation injury model in cultured neonatal rat cardiomyocyte and screen active components from Shexiang Baoxin Pill （ SBP） absorbed in blood against hypoxia/reoxygenation injury .Methods Cardiomyocytes were isolated and purified from hearts of neonatal Sprague Dawley rats （1~3 days old） and were used to build hypoxia/reoxygenation injury model.The components of SBP absorbed in blood were screened by methyl thiazolil tetracolium （MTT） colorimetic method.Results SBP showed significant protective effect against cardiomyocytes hypoxia /reoxygenation injury atthe concentration of 50 μg/ml.Ginsen-oside Rb1, Rb2, bufalin and muscone of twenty components from SBP absorbed in blood also possessed significant protective effect a -gainst cardiomyocytes hypoxia/reoxygenation injury .Conclusion SBP have the protective activity against cardiomyocytes hypoxia /reoxygenation injury , and ginsenoside Rb1, Rb2, bufalin, muscone are the main active components of SBP .This experiment offered basis for further pharmacodynamics and mechanism study of SBP .

关 键 词：麝香保心丸 心肌细胞 缺氧-复氧模型 活性成分筛选 

分 类 号：R972[医药卫生—药品]