hTERC基因在持续性HPV感染的宫颈组织中的表达  

Expression of hTERC gene in cervical tissue of patients with persistent HPV infection

作　　者：马会[1] 舒丽莎[1] 刘云春[1] 吕亚淑 

机构地区：[1]河北北方学院,河北张家口075000

出　　处：《中国妇幼保健》2014年第16期2576-2578,共3页Maternal and Child Health Care of China

基　　金：国家科技部"十一五"重点支撑项目(2008BAI57B01)

摘　　要：目的:研究不同亚型HPV持续感染的宫颈组织中hTERC基因的表达,并讨论hTERC基因作为诊断不同级别宫颈上皮内瘤变标志物的临床意义。方法:采用荧光原位杂交(fluorescent in situ hybridization,FISH)方法,检测20例HPV转阴宫颈组织及70例持续性HPV感染宫颈组织中hTERC基因的表达;并将hTERC基因的扩增情况与组织病理学诊断结果进行比较。结果:hTERC基因在HPV转阴宫颈组织的阳性率为5.00%;在A5/A6、A7、A9及混合感染组的阳性率分别为:33.33%、53.33%、85.00%及50.00%;A9组hTERC基因扩增与对照组、A5/A6组、A7组及混合感染组比较有统计学差异(P<0.05)。hTERC基因在正常宫颈组织或炎症组、CIN1组、CIN2组及CIN3组的阳性率分别为13.79%、33.33%、66.67%及93.75%,hTERC基因的扩增与宫颈病变的程度呈正相关,hTERC基因在正常宫颈组织或炎症及CIN1组中的扩增与CIN2组、CIN3组比较有统计学差异(P<0.05)。结论:hTERC基因的扩增与宫颈感染HPV的亚型相关,hTERC基因的扩增与宫颈病变程度相关。Objective： To study the expression of human tolemerase（ hTERC） gene in cervical tissue of patients with persistent infection of different subtypes of human papillomavirus（ HPV）,and explore the clinical significance of hTERC as a diagnostic marker of different grades of cervical intraepithelial neoplasia（ CIN）. Methods： FISH method was used to detect the expressions of hTERC in cervical tissue specimens of 20 cases without HPV infection（ control group） and 70 cases with persistent HPV infection; hTERC gene amplification and histopathological results were compared. Results： The positive expression rate of hTERC in control group was 5. 00%; the positive expression rates of hTERC in A5 /A6 infection group,A7 infection group,A9 infection group and mixed infection group were 33. 33%,53. 33%,85. 00% and 50. 00%,respectively; there were statistically significant differences in hTERC gene amplification between A9 infection group and control group,A5 /A6 infection group,A7 infection group,mixed infection group（ P〈0. 05）. The positive rates of hTERC gene in normal cervical tissue or cervical inflammation group,CIN Ⅰ group,CIN Ⅱ group and CIN Ⅲ group were 13. 79%,33. 33%,66. 67%and 93. 75%,respectively; there was a positive correlation between hTERC gene amplification and the degrees of cervical lesions,there was statistically significant difference in hTERC gene amplification between normal cervical tissue or cervical inflammation group,CIN Ⅰ group and CIN Ⅱ group,CIN Ⅲ group（ P〈0. 05）. Conclusion： hTERC amplification is related to subtypes of HPV infection,hTERC gene amplification is correlated with the degrees of cervical lesions.

关 键 词：人端粒酶基因 人乳头瘤病毒 荧光原位杂交 宫颈病变 

分 类 号：R711.3[医药卫生—妇产科学]