煨木香醇提物的纯化工艺优选  

Optimization of Purification Technology of Alcohol Extracts of Roasted Aucklandiae Radix

作　　者：战旗[1] 戴兵[1] 王蕊[1] 周广涛[1] 高鹏[1] 

机构地区：[1]山东中医药大学,济南250355

出　　处：《中国实验方剂学杂志》2014年第11期16-19,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技部"重大新药创制"科技重大专项(2013ZX09103002-015)

摘　　要：目的:初步考察煨木香醇提物中内酯类成分及油脂类成分的药理作用,优选煨木香醇提物的纯化工艺。方法:以墨汁推进率、腹泻指数为指标,通过药理试验考察煨木香醇提物经醇溶冷藏后内酯类及油脂类成分对健康小鼠及番泻叶所致小鼠腹泻模型的影响;以木香烃内酯、去氢木香内酯转移率及干膏得率的综合评分为指标,采用正交试验考察乙醇体积分数、乙醇用量、冷藏温度、冷藏时间对冷藏工艺的影响。结果:煨木香醇提物中内酯类成分可减缓小鼠小肠推进运动,降低腹泻指数;油脂类成分能增强小鼠小肠推进运动,显著增加腹泻指数,具有滑肠致泻作用。煨木香醇提物最佳冷藏工艺为加5倍量80%乙醇于-10℃冷藏5 h;木香烃内酯、去氢木香内酯转移率及干膏得率分别为94.9%,93.8%,5.1%。结论:煨木香醇提物中油脂类成分宜除去。优选的冷藏工艺简便易行,在利于制剂成型基础上可除去具滑肠作用的油脂类成分,可推广于实际大生产应用。Objective： To investigate pharmacological action of lactone part and fat part in alcohol extracts of roasted Aueklandiae Radix, then optimize purification technology of the alcohol extracts. Method： Taking ink propulsion rate in the small intestine and diarrhea index as indexes, effects of lactone part and fat part in alcohol extracts of roasted Aucklandiae Radix after cold storage on diarrhea induced by Sennae Folium in healthy mice were investigated. With composite score of yield of dry extract, transfer rates of costunolide and dehydrocostus lactone as index, orthogonal test was adopted to optimize purification technology by taking ethanol concentration and consumption, refrigeration temperature and time as factors. Result： Propulsive motility in the small intestine and diarrhea index could be reduced by lactone part, while fat part had opposite effects with slippery bowel purgative effect. The best purification technology was as follows： refrigerated 5 h with 5 times the amount of 80% ethanol at - 10 ℃ ; yield of dry extract, transfer rates of costunolide and dehydrocostus lactone were 5.1% , 94.9% , 93.8% , respectively. Conclusion： Fat part in alcohol extracts of roasted Aucklandiae Radix should be removed. This optimized refrigeration process was simple and feasible, based on in favor of preparation, it could remove fat part with slippery bowel purgative effect, and had a certain reference value in actual production.

关 键 词：煨木香 冷藏除杂工艺 番茄叶腹泻模型 木香烃内酯 去氢木香内酯 墨汁推进率 稀便率 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]