猪皮胶原肽的分离纯化及结构鉴定  

Separation and Indentification of Collagen Peptides from Pigskin

作　　者：宋景[1] 刘长龙[1] 高鹏[1] 代龙[1] 

机构地区：[1]山东中医药大学,济南250355

出　　处：《中国实验方剂学杂志》2014年第11期23-25,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山东省自然科学基金项目(ZR2011HM051);国家"十一五"科技支撑计划项目(2008BAI53B073)

摘　　要：目的：分离纯化猪皮胶原肽并鉴定其活性成分的相对分子质量分布及结构。方法：采用胃蛋白酶和胰蛋白酶酶解猪皮，酶解液超滤，电渗析除盐，冻干得猪皮胶原肽，利用Sephadex-G25凝胶柱色谱对酶解产物进行分离纯化，分析各部位对L929细胞增值作用的影响，运用ESI-Q-TOF2质谱仪对活性部位进行肽序列分析。结果：分离纯化得到2个活性部位（部位I，Ⅱ），二者对L929细胞的增殖率分别为162％，179％。活性部位Ⅱ相对分子质量主要分布于800～2000Da，首次发现GLGAGGGRGsDGNPG，PAGAHGPAGL共2个序列。结论：猪皮胶原肽具有显著的细胞增殖作用，符合G-X-Y胶原排列组合，具有一定专属性。Objective： To isolate and purity collagen peptides from pigskin, then identify relative molecular mass distribution and structure of its active ingredients. Method： Pigskin was digested by pepsin and trypsin, enzyme solution received uhrafiltration, electrodialysis desalination and lyophilization to get collagen peptides from pigskin, Sephadex G-25 gel column was adopted to separate and purity hydrolyzate, effects of each part of collagen peptides on L929 cell multiplication were analyzed, ESI-Q-TOF2 mass spectrometer was employed to analyze amino acid sequence of collagen peptides from pigskin. Result： Two parts were separated as part I and Ⅱ , both of them on L929 cell multiplication rates were 162% and 179% , respectively. Relative molecular mass of activity part Ⅱ were 800-2 000 Da, two amino acid sequence were first discovered, they were GLGAGGGRGSDGNPG and PAGAHGPAGL proliferation with some specificity, which met Conclusion： Collagen peptides from pigskin had a significant cell permutations of G-X-Y.

关 键 词：猪皮胶原肽 分离纯化工艺 仿生酶解法 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]