HPLC测定新疆产短星火绒草中绿原酸和咖啡酸的含量  

Determination of Chlorogenic Acid and Caffeic Acid in Leontopodium brachyactis from Xinjiang by HPLC

作　　者：龚健[1] 卢帅[1] 索菲娅[1] 王傲立[1] 叶星[1] 

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830046

出　　处：《中国实验方剂学杂志》2014年第11期50-52,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(31160073);新疆维吾尔自治区教育厅项目(XJEDU2010I12);新疆维吾尔自治区科技厅项目(201091245)

摘　　要：目的：建立HPLC测定新疆产短星火绒草中绿原酸和咖啡酸含量的方法，并用于短星火绒草中绿原酸和咖啡酸含量的测定。方法：采用Diamonsil“（钻石）C18色谱柱（4．6mm×250mm，5μm），流动相乙腈-0.5％磷酸溶液（10：90），流速1．0mL·min^-1，柱温40℃，检测波长280nm。结果：绿原酸线性范围0．08～0．76μg（r=0．9999），平均加样回收率99．48％，RSD2．82％。咖啡酸线性范围0．051～0．459μg（r=0．9999），平均加样回收率100．31％，RSD0．96％。结论：该法简单、准确、重复性好，可用于测定短星火绒草中绿原酸和咖啡酸含量。Objective： To establish high performance liquid chromatography （HPLC） method for the determination of chlorogenic acid and caffeic acid in Leontopodium brachyactis from Xinjiang for the first time. Method： The stationary phase was DiamonsilTM Cls （4.6 mm× 250 mm, 5μm）column and the mobile phase was acetonitrile-0. 5% phosphoric acid （ 10： 90）. The flow rate was maintained at 1.0 mL ·min^-1 and the column temperature was set at 40 ℃. The detective wavelength was set at 280 nm. Result： The liner range of chlorogenic acid was 0.08-0. 76 μg （r = 0. 999 9）. The average recovery was 99.48% （ RSD 2.82% ）. The liner range of caffeic acid was 0.051-0.459 μg （r = 0.999 9）. The average recovery was 100.31% （ RSD 0.96%）. Conclusion： The method is simple, accurate and sensible, and can be used to determine the content of the chlorogenic acid and caffeic acid in L. brachyactis.

关 键 词：短星火绒草 绿原酸 咖啡酸 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]