偏最小二乘-紫外分光光度法测定布渣叶中浸出物含量  

Determination of Extracts in Microctis Folium by PLS-UV Spectrophotometry

作　　者：孙冬梅[1,2] 谭志灿[1] 毕晓黎[1,2] 罗文汇[1] 李养学[1] 李素梅[1] 

机构地区：[1]广东省中医研究所,广州510095 [2]广州中医药大学,广州510405

出　　处：《中国实验方剂学杂志》2014年第11期62-65,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省中医药局项目(20111131号);广东省中医药局项目(20121242号)广东省重大科技专项(2012A080202016)

摘　　要：目的:提出一种快速测定布渣叶浸出物含量的新方法。方法:运用偏最小二乘法(PLS)建立紫外光谱(UV)与浸出物含量测定值之间的多元校正模型,对未知样品进行含量预测。结果:校正模型相关系数(R2)为0.964 1。验证集预测平均相对偏差为1.73%。结论:PLS-UV法具有分析速度快、预测结果准确等优点,适合对组成复杂的中药浸出物进行快速分析,可用于布渣叶药材浸出物的快速测定。Objective： To develop a novel method for fast determination of extracts content in Microctis Folium with PLS-UV Spectrophotometry. Method： Multivariate calibration model based on PLS algorithm was developed to correlate the spectra and the corresponding values determined by the reference method. Result： The correlation coefficient （ R2 ） of the calibration models was 0. 964 1, and the prediction average relative deviation for extracts content were 1.73%. Conclusion： PLS-UV technique used in this study is timesaving and accuracy. It is qualified to rapidly analyze the extracts content of traditional Chinese medicine whose components are complex. PLS-UV can be used to determine the extracts content of Microctis Folium.

关 键 词：偏最小二乘法 紫外分光光度法 布渣叶 浸出物 

分 类 号：R284.1[医药卫生—中药学]