不同部位的骆驼刺中总还原糖与芦丁的含量测定  

Dynamic Changes of Rutin and Total Polysaccharides in Alhagi sparsifolin from Different Parts

作　　者：陈良[1] 陈刚 徐晓琴 

机构地区：[1]新疆医科大学附属中医医院,乌鲁木齐830000 [2]新疆维吾尔自治区中药民族药研究所,乌鲁木齐830002

出　　处：《中国实验方剂学杂志》2014年第11期66-69,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：新疆维吾尔自治区科技重大专项项目(200733146-1)

摘　　要：目的：建立测定骆驼刺中总还原糖与芦丁含量的方法，并对不同部位的样品含量进行比较。方法：以硫酸蒽酮法，采用紫外分光光度法测定骆驼刺中总还原糖的含量，以葡萄糖为对照品，检测波长620nm；采用高效液相色谱法测定芦丁的含量，色谱柱Symmetry Shield Rp-18（3．9mm×150mm，5μm），流动相乙腈-0．1％磷酸水溶液（19：81），检测波长360nm，柱温30℃。结果：葡萄糖检测的线性范围3．85～70．33mg·L^-1（r=0．9959），平均加样回收率99．60％，RSD1．5％（n=6）；芦丁检测的线性范围4．95～59．40mg·L^-1（r=0．9996），平均加样回收率99．30％，RSD2．08％。结论：不同部位的骆驼刺中芦丁和总还原糖含量有一定差异，其中果实及地上部分中总还原糖和芦丁的含量比较高。该结果可为骆驼刺的种植和采收的研究奠定基础。Objective： To establiah the methods for determination of total polysaccharides and rutin content in Alhagi sparsifolin, and to compare the content of each sample from the different parts. Method： With glucose as control, total polysaccharides were measured by UV spectrophotometry at detection wavelength of 620 nm by anthrone sulphuric acid colorimetry. The content of rutin was determined by HPLC. The determination was performed on Symmetry shield Rp18 （3.9 mm × 150 mm, 5 μm） column with mobile phase consisted of acetonitrile-0. 1% phosphoric acid solvent （19：81 ） with the detection wavelength at 360 nm and the column temperature at 30℃. Result： The linear range of total polysaccharides was 3.85-70.33 mg ·L^-1 （r = 0. 995 9） , and the linear range of rutin was 4.95-59.4 mg· L^-1 （ r = 0. 999 6）. Total polysaccharides and mtin in A. sparsifolin from different parts had certain differences. Conclusion： A. sparsifolin cultivated in the fruit and in part on the ground harvest in the total polysaccharides and the content of rutin are higher. It will provide foundation for the research for A. sparsifolin cultivation and harvest.

关 键 词：骆驼刺 总还原糖 芦丁 

分 类 号：R284.1[医药卫生—中药学]