柱前衍生HPLC分析黄连多糖的单糖组成  

Analysis of Monosaccharide Compositions of Polysaccharides in Coptidis Rhizoma by Pre-column Derivatization HPLC Method

作　　者：范刚[1] 唐策[1] 李艳[1] 杨永东[1] 张艺[1] 

机构地区：[1]成都中医药大学民族医药学院,成都611137

出　　处：《中国实验方剂学杂志》2014年第11期74-78,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省教育厅项目(13ZB0315);成都中医药大学科技发展基金项目(ZRMS201342)

摘　　要：目的:建立柱前衍生HPLC测定黄连多糖中的单糖组成,并分析3种黄连多糖的单糖差异。方法:采用水提醇沉法提取黄连多糖,经硫酸水解后,用1-苯基-3-甲基-5-吡唑啉酮(PMP)柱前衍生,再利用HPLC法分析单糖的PMP衍生物。结果:黄连多糖主要由甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖7种单糖组成,其中半乳糖醛酸含量最高,葡萄糖次之。另外,雅连多糖、云连多糖和味连多糖中的7种单糖的平均摩尔比分别为1∶2.34∶0.38∶17.58∶12.40∶6.11∶5.93,1∶2.43∶0.25∶16.76∶15.18∶6.51∶9.78和1∶3.25∶0.40∶22.35∶12.96∶6.66∶10.28。结论:建立的方法简便、准确,重复性好,可用于黄连多糖中单糖的组成分析和含量测定。雅连多糖、云连多糖和味连多糖中的7种单糖含量有差异。Objective： To establish a precolumn derivation HPLC method for the determination of monosaccharide compositions in Coptidis Rhizoma polysaccharides, and analyze the monosaccharides differences between three Coptidis Rhizoma polysaccharides. Method： The polysaccharides were extracted by hot distilled water, precipitated by alcohol, and hydrolyzed into monosaccharides with 2 mol·L^-1 sulfuric acid. The hydrolysate was derivatized with PMP, and then the PMP derivates of monosaCcharides were analyzed by HPLC method. Result： The Rhizoma Coptidis polysaccharides were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose. Among them, the content of galacturonic acid was the most abundant, followed by glucose. In addition, the average molar ratio of seven monosaccharides of C. deltoidea polysaccharide, C. tecta polysaccharide and C. chinensis polysaccharide were 1：2.34： 0.38： 17.58： 12.40：6. 11： 5.93, 1：2.43：0.25： 16.76： 15. 18：6.51：9.78 and 1：3.25：0.40：22.35： 12.96：6.66： 10.28, respectively. Conclusion： The established method in this study is simple, accurate, reproducible, and can be used for the analysis of monosaeeharide compositions of Coptidis Rhizoma polysaceharides. The content of seven monosaeeharides of C. deltoidea polysaeeharide, C. tecta polysaceharide and C. chinensis polysaccharide are different.

关 键 词：黄连多糖 柱前衍生 单糖 云连 雅连 味连 

分 类 号：R284.1[医药卫生—中药学]