烧伤愈合软膏及其水凝胶中3种小檗碱型生物碱的药动学分析  

Pharmacokinetics of Three Alkaloids in Burn Healing Creams and Its Hydrogels

作　　者：徐彬[1,2] 陈永刚 邹吉利 吴金虎 

机构地区：[1]武汉市第三医院药学部,武汉430060 [2]湖北中医药大学药学院,武汉430065

出　　处：《中国实验方剂学杂志》2014年第11期99-102,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：湖北省科技计划自然科学基金(2011CDB298)

摘　　要：目的：建立大鼠血浆中盐酸黄连碱、盐酸巴马汀和盐酸小檗碱的HPLC测定，比较烧伤愈合软膏及其水凝胶中3种生物碱的生物利用度。方法：制备大鼠深二度烫伤模型，创面分别按0．02g·g^-1剂量给予烧伤愈合软膏及其水凝胶，通过HPLC测定不同采血点血浆中3种生物碱的含量，采用PKsolver 2．0软件拟合求算药动学参数。结果：3种生物碱的药一时曲线呈非房室模型，水凝胶组中生物碱在大鼠体内的生物利用度优于烧伤愈合软膏组，水凝胶组中3种生物碱的Cmax提高了1．2～2．6倍，AUC0-24h提高了1．2～1．7倍。结论：建立的HPLC简单、可靠，可用于小檗碱型生物碱血药浓度分析及其药代动力学分析；烧伤愈合软膏及其水凝胶经皮吸收迅速，释放缓慢。Objective： To establish a sensitive and specific HPLC method for determination of plasma concentrations of three alkaloids in rats plasma and compare their bioavailabilities in burn healing ointments and hydrogels. Method： Deep Ⅱ scald model of rats was established, wound were given burn healing ointments and hydrogels with 0.02 g·g^-1, respectively. Then plasma concentrations of three alkaloids at different time points were determined by HPLC and major pharmacokinetic parameters were estimated by PKsolver 2.0 software. Result： Concentration-time curves of three alkaloids conformed to a atriovertricular model, in vivo bioavailability of three alkaloids in hydrogels group were superior to ointments group, compared to the latter, the former existed 120%-260% increases in Crux and 120%-170% increases in AUC0-24 h. Conclusion： This established HPLC method was simple and sensitive to determine concentrations of three alkaloids in rats plasma and could be used for pharmaeokinetie analysis; it could find that two dosage forms were quickly absorded and slowly released.

关 键 词：烧伤愈合软膏 水凝胶 生物碱 药代动力学 深二度烫伤模型 盐酸小檗碱 

分 类 号：R283.6[医药卫生—中药学] R945[医药卫生—中医学]