八角莽草酸的抗动脉粥样硬化作用机制研究  

Mechanism of Anti-atherogenesis of Shikimic Acid from Illicium verum

作　　者：黄晓婷[1] 杨冬业[1] 郑楚[1] 范志敏[1] 刘漫君[1] 韦桂宁[2] 

机构地区：[1]桂林医学院,广西桂林541001 [2]广西中医药研究院,南宁530022

出　　处：《中国实验方剂学杂志》2014年第11期126-130,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西自然科学基金项目(2010GXNSFA013255)

摘　　要：目的:探讨八角莽草酸对动脉粥样硬化(AS)大鼠抗氧化系统、炎症因子的水平及ⅡA分泌型血小板型磷脂酶A2(sPLA2-ⅡA)mRNA表达的影响,以了解莽草酸对动脉粥样硬化的作用机制。方法:健康SPF级雄性Wistar大鼠72只,随机分正常对照组、动脉粥样硬化模型组、阳性(辛伐他汀,5 mg·kg-1)对照组、莽草酸低、中、高剂量组(15,30,60 mg·kg-1),高脂饲料加腹腔注射维生素D3法复制动脉粥样硬化模型,灌胃给以莽草酸,24周后,观察莽草酸对模型大鼠血清超氧化物歧化酶(SOD)活性、丙二醛(MDA)、C反应蛋白(CRP)和血清肿瘤坏死因子α(TNF-α)的水平,主动脉弓sPLA2-ⅡA mRNA表达的影响。结果:与正常组比较,模型组大鼠血清TNF-α,CRP,MDA水平显著增高,SOD的活性显著降低,sPLA2-ⅡA mRNA的表达显著增强。与模型组比较,莽草酸60 mg·kg-1剂量组可显著降低AS大鼠血清TNF-α,CRP水平,分别由模型组的(49.88±22.91)ng·L-1,(10.82±4.33)mg·L-1下降到(28.18±15.95)ng·L-1,(8.17±1.74)mg·L-1(P<0.05)。莽草酸60 mg·kg-1剂量组可显著降低AS大鼠血清MDA水平,由模型组的(21.55±4.45)μmol·L-1下降到(18.70±1.95)μmol·L-1(P<0.05),可提高SOD的活性,由模型组的(145.81±50.61)U·mL-1提高到(199.87±50.29)U·mL-1(P<0.05);莽草酸60,30,15 mg·kg-1剂量组显著下调AS大鼠主动脉弓sPLA2-ⅡA mRNA的表达(P<0.01)。结论:莽草酸可改善AS大鼠炎症状态,且有抗氧化及抑制sPLA2-ⅡA mRNA表达的作用。Objective： To investigate the effects of shikimic acid on antioxidation ability, inflammatory factors and secretory platelet phospholipase A2 of group Ⅱ A （ sPLA2- Ⅱ A） mRNA in atherosclerosis rats. Method： Seventy-two Wistar rats （n = 12） were randomly divided into six group, the normal goup, the model group, the Simvastatin group, high shikimic acid group （60 mg ·kg^-1 ） , middle shikimic acid group （30 mg · kg^-1） and low shikimic acid group （15 mg ·kg^-1）. Atherosclerosis rats were induced by injected with asingle dose of vitamin D （6 × 105 U ·kg^-1） and loaded with high fat diet in order to copy the model. After 24 weeks administration, the serum superoxide-dismutase （SOD）, malondialdehyde （MAD）, tumor necrosis factor-α （TNF-α） and sPLA2-Ⅱ A mRNA were observed. Result ： Compared with normal control group, the serum level of shikimic acid of TNF-α, CRP, MDA, the expression of sPLA2 mRNA of model group was remarkably increased, the actixity of SOD were inhibited （P 〈 0.01, P 〈 0.05）. Shikimic acid could reduce the level of serum CRP and TNF-α from （10.82±4.33） mg·L^-1 and （49.88 ±22.91） ng·L^-1 of the model group to （8.17±1.74） mg·L^-1 and （28. 18 ± 15.95） ng·L^-1 （P 〈0.05）. Reduce the level of serum MDA from （21.55±4.45） μmol· L^-1 of the model group to （18.70 ± 1.95） μmol ·L^-1 （P 〈0.05）, and improve the activity of SOD from （145.81 ±50.61） U·mL^-1 of the model group to （199.87±50.29） U ·mL^-1 （P 〈0.05）, and inhibit the expression of sPLA2- Ⅱ A mRNA （P 〈 0.01 ）. Conclusion： Shikimie acid can reduce the level of inflammatory factors, improve antioxidation ability, and inhibit the expression of sPLA2- Ⅱ A mRNA.

关 键 词：八角莽草酸 动脉粥样硬化 抗氧化 炎症因子 ⅡA分泌型血小板型磷脂酶A2 

分 类 号：R285.5[医药卫生—中药学]