甘草泻心汤配方颗粒剂对复发性口腔溃疡模型大鼠的作用  

Effect of Gancao Xiexin Decoction Formula Granules on Model Rats with Recurrent Oral Ulcer

作　　者：王金凤[1] 刘文辉[1] 荆雪宁[1] 陈莉[1] 王枫[1] 

机构地区：[1]山东中医药高等专科学校,山东烟台264199

出　　处：《中国实验方剂学杂志》2014年第11期143-146,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山东省中医药科技发展计划项目(2011-149)

摘　　要：目的:研究甘草泻心汤配方颗粒剂对复发性口腔溃疡(ROU)模型大鼠的作用。方法:Wister大鼠48只,随机分为6组:正常组、模型组、左旋咪唑(17.5 mg·kg-1)组、甘草泻心汤配方颗粒剂高、中、低剂量(12,6,3 g·kg-1)组。除正常组外,余组大鼠均采用免疫法建立ROU大鼠模型。各组分别连续ig给药20 d后,热板法检测大鼠痛阈,酶联免疫吸附法(ELISA)检测大鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-8(IL-8)的含量,流式细胞仪测定大鼠外周血T淋巴细胞亚群。结果:与正常组比较,模型组大鼠痛阈下降,血清TNF-α,IL-8含量升高,外周血T淋巴细胞亚群CD4+细胞数下降CD8+细胞数上升,CD4+/CD8+下降(P<0.01)。与模型组比较,甘草泻心汤配方颗粒剂高、中剂量组均可提高大鼠痛阈:(4.1±0.7),(9.2±2.0),(8.5±1.4)s,降低血清TNF-α含量(182.4±11.6),(37.8±9.0),(42.7±4.6)ng·L-1,降低血清IL-8含量(870.0±42.2),(303.7±31.7),(331.8±18.6)ng·L-1,升高CD4+细胞(37.4±3.6)%,(48.0±1.5)%,(48.1±4.4)%,降低CD8+细胞数(32.1±3.6)%,(26.3±2.7)%,(26.8±1.2)%,提高CD4+/CD8+(1.2±0.1),(1.8±0.3),(1.8±0.2),(P<0.01)。结论:甘草泻心汤配方颗粒剂对复发性口腔溃疡模型大鼠有较好的治疗作用,可能与其镇痛、抑制炎症因子和调节T淋巴细胞亚群失衡作用有关。Objective： To study the effect of Gancao Xiexin decoction formula granules on model rats with recurrent oral ulcer （ROU）. Method： Forty-eight Wister rats were randomly divided into six groups： normal group, model group, levamisole group （17.5 mg·kg^-1） , three Gancao Xiexin decoction formula granules groups- high, medium and low （12, 6, 3 g·kg^-1）. Except the rats in the normal group, the rats in other groups were used to establish the recurrent oral ulcer model with immune method. After 20 days of continuous intragastric administration, the pain threshold in rats was tested by hot plate method, the tumor necrosis factor-α （TNF-α） and interleukin-8 （IL-8） levels in rat serum were tested by enzyme-linked immunosorbent assays （ELISA）, and T lymphocyte subsets of peripheral blood was determined by flow cytometry. Result： Compared with normal group, the pain threshold of rats in the model group was decreased, and the TNF-α and IL-8 levels in rat serum were increased, the number of CD4^＋ cells fell, and the number of CD8^＋ ceils rose, CD4^＋/CD8^＋ ratio decreased （P 〈 0.01 ）. Compared with model group, in the high and medium Gancao Xiexin decoction formula granules groups, the pain threshold was increased： （4.1±0.7）, （9.2 ±2.0）, （8.5±1.4） s, the TNF-α in rat serum was decreased： （182.4 ±11.6）, （37.8 ±9.0）, （42.7 ±4.6） ng·L^-1, and IL-8 was decreased： （870.0± 42.2）, （303.7 ±31.7）, （331.8±18.6） ng·L-1, the number of CD4^＋ cells rose： （37.4±3.6）%, （48.0±1.5）%, （48. 1±4.4）%, and the number of CD8^＋ cells fell： （32.1 ±3.6）%, （26.3±2.7）%, （26.8± 1.2）%, leading to an increased CD4^ ＋/CD8 ^＋ ratio： （1.2±0.1）, （1.8±0.2）, （1.8 ＋0.2） （P〈0.01）. Conclusion： Gancao Xiexin decoction formula granules is effective in the treatment of model rats with recurrent oral ulcer, which may have something to do with its effects of analgesia, inhibiting inflammatory factors, a

关 键 词：甘草泻心汤 配方颗粒剂 复发性口腔溃疡 痛阈 细胞因子 T淋巴细胞亚群 

分 类 号：R285.5[医药卫生—中药学]