复智胶囊对血管性痴呆模型大鼠海马神经元突触传递长时程增强的影响  

Effect of Fuzhi Capsule on Long-term Potentiation of Hippocampus Neuronal Cell of Rats with Vascular Dementia

作　　者：张杰[1,2] 马云枝[2] 朱筱彬[3] 杨泽锋[3] 刘政伟[3] 

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]河南中医学院第一附属医院,郑州450008 [3]河南中医学院,郑州450008

出　　处：《中国实验方剂学杂志》2014年第11期155-159,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省中医药科研专项课题(2013ZY01011);河南省科技创新人才基金项目(094200510012)

摘　　要：目的:观察复智胶囊对血管性痴呆大鼠海马神经元突触传递长时程增强(long-term potentiation,LTP)的影响。方法:SPF级雄性SD大鼠采用双侧颈总动脉永久结扎法(2-VO)制备血管性痴呆大鼠模型。选取造模成功的大鼠,随机分为模型组、多奈哌齐(0.52×10-3g·kg-1·d-1,ig)组、复智胶囊高、低剂量(6.30,3.15 g·kg-1·d-1,ig)组。并设假手术对照组。连续ig 4周后,采用Morris水迷宫检测实验大鼠的学习记忆能力,同时采用LTP诱导法检测大鼠海马齿状回高频刺激诱发的群峰电位(population spike,PS)幅值变化,采用免疫组化检测海马N-甲基-D-天门冬氨酸(NMDA)免疫阳性细胞的表达。结果:与假手术组相比,造模术后的大鼠学习记忆能力均有下降,逃避潜伏期和游泳路程明显延长,撤除平台后跨越原平台次数明显减少,高频电刺激后海马神经元齿状回PS增幅明显下降,海马NMDA免疫阳性细胞数明显减少,差异均有统计学意义(P<0.05);而与模型组相比,复智胶囊高、低剂量组和多奈哌齐组大鼠学习记忆能力均有改善,逃避潜伏期和游泳路程均有缩短,跨越原平台次数明显增多,海马神经元齿状回PS增幅明显升高,海马NMDA免疫阳性细胞数明显增多,差异均有统计学意义(P<0.05)。结论:复智胶囊通过增加海马NMDA阳性细胞表达,提高海马神经元齿状回PS增幅,改善大鼠学习记忆能力,对血管性痴呆模型大鼠海马神经元突触传递长时程增强有明显的改善作用。Objective： To observe the Fuzhi Capsule on long-term potentiation of hippocampus neuronal cell of rats with vascular dementia. Method： The SPF male SD rats were divided into model group, Donepezil group, Fuzhi capsule high and low dosage groups, and sham operation control group randomly. The vascular dementia model was established by permanent bilateral common carotid artery ligation （2-VO）. Drugs were administered to rats by gastric perfusion once a day for 4 weeks. Then all the rats were detected the behavior by Morris water maze, the changes of population spike potential amplitude induced by the high frequency stimulation will be detected by long-term potentiation （LTP） inducted method and the expression of N-niethyl-D-asportate （NMDA） in hippocampus by immunohistochemistry. Result： Compared with the sham operation group, the learning and memory ability of rats in model group were decreased, the escape latency and swimming distance were significantly prolonged, the number of across the platform after remove platform were decreased, the population spike potential amplitude of hippocampal neurons in dentate gyrus decreased markedly, the number of NMDA immunoreactive cells was significantly reduced （P 〈 0.05 ）. Compared with the model group, the learning and memory ability of rats in Fuzhi capsule high and low dosage group and donepezil group were improved, the escape latency and swimming distance were shortened, the number of across the platform after remove platform were increased obviously, the population spike potential amplitude of hippocampal neurons in dentate gyrus increased markedly, the number of NMDA immunoreactive cells was significantly increased （P 〈 0.05）. Conclusion： Fuzhi capsule can improve the learning and memory ability of rats with vascular dementia by increasing the number of NMDA immunoreactive cells and the population spike potential amplitude of hippocampal neurons, improve the long term potentiation of synapses of hippocampal neurons of rat with vascular d

关 键 词：血管性痴呆 复智胶囊 学习记忆能力 N-甲基-D-天门冬氨酸 长时程增强 

分 类 号：R285.5[医药卫生—中药学]