通脉地仙丸对大鼠缺血心肌血管新生的作用  

Effect of Tongmai Dixian Pills on Angiogenesis of Ischemic Myocardium

作　　者：栾杰男[1] 张大维[2] 焦燕[2] 李根[1] 巩琪[3] 

机构地区：[1]深圳市中医院,广东深圳518033 [2]吉林大学白求恩医学院,长春130021 [3]广东医学院附属南山人民医院,广东深圳518033

出　　处：《中国实验方剂学杂志》2014年第11期170-174,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省自然科学基金项目(9151803301000007)

摘　　要：目的:通过通脉地仙丸作用于心肌缺血的大鼠,观察缺血心肌梗死边缘区的微血管新生情况,初步探讨其心肌保护机制。方法:100只雄性大鼠慢性心肌缺血造模成功后除假手术组外随机分为模型组,通脉地仙高、中、低(6.3,3.15,1.57 g·kg-1)剂量组,阳性药麝香保心丸(0.014 2 g·kg-1)组。灌胃给药6周后观察大鼠对心肌梗死范围的影响,同时苏木精-伊红(HE)染色观察心肌组织病理形态学的改变,并免疫组织化学检测观察心肌梗死边缘区微血管新生情况。结果:①与模型组比较,通脉地仙高、中剂量组和麝香保心丸组心肌梗死面积不同程度减小(P<0.05,P<0.01)。②通脉地仙丸对大鼠心肌组织的影响:光镜下观察,假手术组心肌纤维排列整齐且无肿胀现象,心肌间质极少炎性细胞浸润;模型组心肌纤维肿胀,排列紊乱,心肌间质大量炎细胞浸润,细胞核大小不一,甚至可见局灶性坏死区;通脉地仙高、中剂量组心肌细胞核大小均一,心肌纤维轻度肿胀,排列比较整齐,仍可见不同程度的成纤维细胞及炎细胞浸润;阳性对照组镜下心肌纤维排列基本整齐,轻度肿胀,可见少量炎细胞浸润。③缺血心肌血管新生的微血管密度(MVD)比较:与假手术组比较,模型组大鼠微血管密度相对增加(P<0.05),通脉地仙高、中剂量组和阳性对照组与模型组比较有明显微血管密度增加的趋势(P<0.01),通脉地仙低剂量组MVD也有不同程度的增加微血管的作用,但与模型组比较没有统计学差异。结论:通脉地仙丸可以通过促进心梗边缘区微血管的生成从而达到保护心肌组织和细胞的目的。Objective： This study is to observe the effect of Tongmai Dixian pills on the angiogenesis of ischemic myocardium border zone and to research myocardial protection mechanisms. Method： One hundred male rats with chronic myocardial ischemia model except the sham group, which were randomized, model group, Tongmai Dixian pills high, middle and low （6. 3, 3.15, 1.57 g·kg^-1 ） dose groups, and Shexiang Baoxin pills （0. 014 2 g ·kg^-1） group. Intragastric administration for six weeks, hematoxylin-eosin （HE） staining of myocardial pathomorphology changes and immunohistochemical staining observed neovascularization of myocardial infarct border zone. Result： ①Tongmai Dixian pills can deduce the scope of myocardial infarctions as to protect the heart. ②Tongmai Dixian pills on the myocardial tissue （HE staining） ： sham group＇s the cardiac muscle fibers arranged in neat rows and inflammatory cells infiltration; model group＇s cardiocytes had serious edema, lots of disorganized myocardial interstitial inflammatory cells infiltration, or even visible its focal necrosis; Tongmai Dixian pills each dose group myocardial nucleus size is uniform, showing varying degrees of fibroblasts and inflammatory cells infiltration; Shexiang Baoxin pills group under the light microscopic is similar to Tongmai Dixian pills middle dose group; ③ischemic myocardial angiogenesis microvessel density （MVD）： compared with the sham group, model group with significant difference （ P 〈 0.05 ） , Tongmai Dixian pills each dose group can be significantly increased （P 〈 0.05 or P 〈 0.01 ） , Tongmai Dixian pills high, middle dose and Shexiang Baoxin pills can also be significantly increased （P 〈0.01）, Tongmai Dixian pills low dose group is also an increasing trend, but no statistically significant differences compared with model group. Conclusion： Tongmai Dixian pills can promote the generation microvascular in the myocardial infarction border zone in order to achieve the purpose of protecting the

关 键 词：通脉地仙丸 微血管密度 心肌缺血 

分 类 号：R285.5[医药卫生—中药学]