灯盏花素对对比剂急性肾损伤大鼠肾脏Na^+,K^+-ATP酶活性的影响  

Effects of Breviscapine on the Na+,K+-ATPase activity in kidney tissues of rats with contrast-induced acute kidney injury

作　　者：陈子洁 石理[2] 陈曦[2] 姜小飞[2] 

机构地区：[1]广东省珠海市人民医院干部保健老年病科,519000 [2]广东省珠海市人民医院心内科,519000

出　　处：《广东医学》2014年第7期978-981,共4页Guangdong Medical Journal

基　　金：广东省中医药局建设中医药强省立项课题(编号:20112040)

摘　　要：目的通过观察灯盏花素对对比剂诱导的急性肾损伤大鼠肾脏Na+,K+-ATP酶活性的影响,探讨灯盏花素对对比剂肾损伤大鼠早期的保护作用机制。方法采用健康成年SD大鼠及糖尿病大鼠模型,设正常对照组(NS组)、对比剂对照组(CM组)、灯盏花素治疗组(Bre组)及糖尿病对比剂对照组(DCM组)、糖尿病灯盏花素治疗组(DBre)。NS组予10 mL/kg生理盐水尾静脉注射,其余各组予等量低渗对比剂。NS和CM组再予20mg/(kg·d)腹腔注射、Bre组予等量灯盏花素。一次性腹腔注射链脲佐菌素60 mg/kg建立糖尿病模型,成模糖尿病大鼠注射低渗对比剂后分别予生理盐水及灯盏花素腹腔注射。免疫组化SABC法检测Na+,K+-ATP酶在大鼠肾脏的定位和表达,观察Na+,K+-ATP酶活性变化。结果灯盏花素可使注射对比剂后受抑制的Na+,K+-ATP酶活性表达明显上调(P<0.05),这种作用在糖尿病模型组大鼠(DCM组与DBre组)表现更明显(P<0.01)。结论灯盏花素对大鼠对比剂急性肾损伤有一定的保护作用,该作用可能与减轻对比剂对Na+,K+-ATP酶活性的抑制作用有关。Objective To study the effects of Breviscapine on the Na^＋,K^＋-ATPase activity in kidney tissues of rats with contrast-induced acute kidney injury.Methods SD rats and diabetic rat models were randomly divided into five groups：normal control group（ Group NS）,contrast media control group（ Group CM）,Bre treatment group（ Group Bre）,diabetic model with contrast media group（ Group DCM）,and diabetic model with Bre treatment group（ Group DBre）.Localization and activity of Na^＋,K^＋-ATPase in kidney tissues was detected by immunohistochemistry staining method.Results The activity of Na^＋,K^＋-ATPase in kidney tissues,which was inhibited by contrast media,was significantly enhanced with breviscapine（ P〈0.05）,and it was significantly more prominent in diabetic rats（ Group DCM and DBre）（ P〈0.01）.Conclusion Breviscapine provides a certain protection on contrast-induced acute kidney injury in rats,probably via lightening the inhibition of Na^＋,K^＋-ATPase activity caused by contrast media.

关 键 词：对比剂 急性肾损伤 灯盏花素 NA^+ K^+-ATP酶 

分 类 号：R996.3[医药卫生—毒理学]