山楂叶总黄酮对脑缺血大鼠脑组织PI3K/Akt信号通路的影响  

The effects of Hawthorn leaves flavonoids on the cerebral PI3K/Akt signaling pathway in rats with cerebral ischemia

作　　者：赵蕾[1] 张晓红[2] 吴柏成[3] 吴晓光[4] 杜娈英[1] 陈晓宁[1] 

机构地区：[1]承德医学院病原生物学教研室,河北承德067000 [2]承德医学院影像学教研室,河北承德067000 [3]承德医学院后勤集团饮食服务中心,河北承德067000 [4]承德医学院基础医学研究所,河北承德067000

出　　处：《广东医学》2014年第7期982-984,共3页Guangdong Medical Journal

基　　金：河北省医学科学研究重点课题(编号:20120160)

摘　　要：目的探讨山楂叶总黄酮对脑缺血大鼠脑组织PI3K/Akt信号通路的影响。方法雄性SD大鼠随机分为假手术组、模型组、银杏叶片组和山楂叶总黄酮组,每组20只。后3组采用双侧颈总动脉结扎法制备慢性脑缺血大鼠模型,银杏叶片组给予银杏叶片5.2 mg/kg,山楂叶总黄酮组给予山楂叶总黄酮140 mg/kg灌胃,模型组和假手术组给予等量的生理盐水。免疫组化法检测PI3K、Akt蛋白的表达,干湿重法检测脑组织水含量,甲酰胺法测定血脑屏障通透性。结果与模型组比较,银杏叶片组和山楂叶总黄酮组PI3K、Akt蛋白均明显升高(P<0.05,P<0.01),脑组织含水量及伊文思蓝含量显著降低(P<0.05,P<0.01)。结论山楂叶总黄酮对缺血性脑损伤具有保护作用,其作用机制可能与调控PI3K/Akt信号通路有关。Objective To study the effects of Hawthorn leaves flavonoids（ HLF） on the PI3K/Akt signaling pathway in rats with cerebral ischemia.Methods Chronic cerebral ischemia rat model was constructed by bilateral carotid artery ligation.PI3K and Akt protein expression was assessed by immunohistochemical assay.Water content of brain tissue was detected by dry-wet method,while brain barrier permeability was measured by formamide method blood.Results Compared with the model group,significant up-regulations in PI3K and Akt proteins were observed in Ginkgo biloba group and HLF group（ P〈0.05）.Significant reductions in cerebral water content and EB content were also observed（ P〈0.05）.Conclusion HLF can protect the brain from ischemia injury,probably via regulation of PI3K/Akt signaling pathway.

关 键 词：山楂叶总黄酮 PI3K/AKT信号通路 脑缺血 

分 类 号：R335.6[医药卫生—人体生理学]