糜酶对犬血管平滑肌细胞增殖的影响  

Effects of chymase on proliferation of cultured dog vascular smooth muscle cells

作　　者：孙成林[1] 马骏[1] 刘宗凤[1] 白英奎[1] 赵延鹏[1] 张绍军[1] 

机构地区：[1]沈阳医学院附属中心医院普外一科,沈阳110024

出　　处：《广东医学》2014年第8期1143-1145,共3页Guangdong Medical Journal

基　　金：辽宁省科学技术计划项目(编号:2011225020)

摘　　要：目的探讨糜酶途径对犬血管平滑肌细胞(VSMC)的血管紧张素Ⅱ(AngⅡ)生成及细胞增殖的作用。方法通过组织块贴壁法培养犬动脉VSMC,分为4组:对照组、AngⅠ组、卡托普利组,糜酶抑素组。对照组中仅为空白培养液,其他各组均加入AngⅠ,卡托普利组和糜酶抑素组再分别加入相应的干预药物。应用台盼蓝染色法和MTT法检测各组VSMC增殖的变化,放射免疫法检测培养液中AngⅡ含量等。结果成功培养出犬VSMC,卡托普利组和糜酶抑素组细胞计数及吸光度值均显著低于AngⅠ组(均P<0.05),而且糜酶抑素组较卡托普利组更明显(P<0.05);糜酶抑素组AngⅡ含量较AngⅠ组和卡托普利组低(P<0.05)。结论糜酶在犬VSMC的AngⅡ生成及细胞增殖过程中起着重要作用,其抑制剂可抑制VSMC增殖。Objective To investigate the effect of chymase on the cell proliferation and angiotensin Ⅱ（ Ang Ⅱ） generation of dog vascular smooth muscle cells. Methods The dog artery smooth muscle cells were cultured and divided into four groups： Control group,Angiotensin Ⅰ（ Ang Ⅰ） group,Captopril group and Chymostatin group. Cell counting was performed by trypan blues staining,while the ratio of light density by MTT and Ang Ⅱ concentration were measured in the culture medium. Results The cell counts were significantly reduced in Captopril group and Chymostatin group compared with Ang Ⅰ group（ P〈0. 05）. The cell proliferation of Chymostatin group was significantly inhibited（ P〈0. 05）. Moreover,the concentration of Ang Ⅱ in Chymostatin group was reduced significantly（ P〈0. 05）. Conclusion Chymase may play an important role in the generation of Ang Ⅱ and the proliferation of cultured dog vascular smooth muscle cells, via which chymostatin may inhibit cell proliferation.

关 键 词：糜酶 犬血管平滑肌细胞 血管紧张素Ⅱ 细胞增殖 

分 类 号：R972[医药卫生—药品]