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作 者:李爽[1] 刘侨博[1] 耿峰[1] 左金龙[2] 谭冲[2]
机构地区:[1]黑龙江省环境保护科学研究院,黑龙江哈尔滨150056 [2]哈尔滨商业大学环境工程系,黑龙江哈尔滨150076
出 处:《中国给水排水》2014年第11期24-28,共5页China Water & Wastewater
基 金:黑龙江省自然科学基金资助项目(E201355)
摘 要:应用单级聚氨酯生物膜反应器处理实际生活污水,考察不同DO浓度下微生物群落结构与其处理效果的关系。经过160d的稳定运行,在不同DO浓度下,聚氨酯生物膜系统内均发生了同步硝化反硝化。DO为0.5~1.0mg/L时得到最大氮去除率(70.6%),其中的72.9%由同步硝化反硝化完成。通过分析氮浓度,亚硝酸盐氮的最大积累量发生在DO为0.5~1.0mg/L时。对生物膜上功能微生物的分析表明,DO为1.5—2.5mg/L时的微生物多样性要显著高于其他工况。PCR—DGGE分析结果表明,优势亚硝化菌和硝化菌分别以Nitrosomonas sp.和Nitrospira sp.为主,而反硝化菌则是Thauera sp.和Pseudomonas sp.占据优势。Microbial community structure and nutrient removal performance at different dissolved oxygen concentrations were investigated using one-step polyurethane biofilm reactor for municipal wastewater treatment. At different dissolved oxygen concentrations, the nitrification and denitrification processes were carried out simultaneously in the polyurethane biofilm reactor after 160 days. The maximal nitrogen removal rate was 70.6% at the DO of 0.5 to 1.0 mg/L, of which 72.9% was achieved by sim- ultaneous nitrification and denitrification. By analyzing the nitrogen species concentration data, it was indicated that nitrite accumulation took place at the DO of 0.5 to 1.0 mg/L. The microbial biofilm analysis showed that microbial diversity of polyurethane biofilm at the DO of 1.5 to 2.5 mg/L was significantly higher than that in other conditions. The PCR-DGGE analysis revealed the dominant nitrite and nitrate bacteria were Nitrosomonas sp. and Nitrospira sp. respectively, and the dominant denitrifying bacteria were Thauera sp. and Pseudomonas sp.
关 键 词:单级自养脱氮 DO 聚氨酯 PCR—DGGE 微生物群落结构
分 类 号:X703[环境科学与工程—环境工程]
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