利用多模式填料降低治疗用单克隆抗体中的酸性组分  

Using Multiple Modes Resin Reduce Acidic Components in Monoclonal Antibodies

作　　者：许峰[1] 章良[1] 

机构地区：[1]苏州大学药学院,江苏苏州215000

出　　处：《广东化工》2014年第10期57-58,共2页Guangdong Chemical Industry

摘　　要：在本项目所生产的治疗性单克隆抗体的生产过程中,由于天冬酰胺在一定条件下分解为天冬氨酸及异天冬氨酸、导致产品的酸性组分高于标准品。因此需要在纯化过程中将其降低,由于其电荷差异小,在生产规模下,无法安装具有足够分辨率的离子交换层析柱来分离酸性组分,因此利用带有疏水集团的多模式填料Capto adhere并调整层析条件,使酸性组分带有一定的疏水性而被填料吸附,达到降低酸性组分的目的。During the production process of the therapeutic monoclonal antibodies in this project, under certain conditions, asparagines decompose to L-Asp and L-isoAsp, and thus the acid components of the product are higher than a standard. Therefore, the acid components need to removed by purification process, due to the small difference between their charges, in the scale of production, it is impossible to install ion exchange chromatography column with sufficient resolution to separate the acidic component, thus using the multi-mode resin Capto adhere having a hydrophobic group adsorb the acidic also hydrophobic components in suitable chromatographic conditions.

关 键 词：多模式填料 降低 酸性组分 

分 类 号：TQ[化学工程]