应用多联PCR对引发猪繁殖障碍有关病毒的检测Ⅱ.JEV、PPV、PRRSV、PRV单项PCR检测方法的建立  

Establishment of Single PCR for JEV,PPV,PRRSV and PRV

作　　者：孙明[1] 李红卫[1] 高显明 涂长春[1] 何旭玉 白晓鸿 金宁一[1] 殷震[1] 

机构地区：[1]解放军军需大学军事兽医研究所,吉林长春130062 [2]北京军区卫生防疫队,北京100071

出　　处：《中国兽医学报》2001年第1期10-13,共4页Chinese Journal of Veterinary Science

摘　　要：在利用计算机设计出检测流行性乙型脑炎病毒 ( JEV)、猪细小病毒 ( PPV)、猪繁殖与呼吸综合征病毒( PRRSV)、猪伪狂犬病病毒 ( PRV)的多联 PCR引物的基础上 ,分别进行各单项 PCR检测相应病毒试验 ,确定了各单项 PCR反应条件范围 ,并对各 PCR扩增产物进行了点杂交和部分测序鉴定。用单项 PCR对感染猪相关病毒的检测证实 ,本试验已经建立了有效、特异、敏感的检测 JEV、PPV、PRRSV、PRV的单项 PCR技术 ,为进一步建立多联After multiple PCR primers for JEV,PPV,PRRSV and PRV was designed by computer,the optimal conditions of single PCR were determined by orthogonal assay,the products of single PCR were characterized with dot blot using oligoneucleic probes and sequenced to be specific.Proved that the methods of single PCR for JEV,PPV,PRRSV and PRV were a simple,sensitive,specific technique, which would favour the further establishment multiple PCR and its application in detection of correspondent viruses in pig reproductive failure.

关 键 词：JEV PPV PRRSV PRV PCR 猪 繁殖障碍 病毒 检测方法 

分 类 号：S854.43[农业科学—临床兽医学] S858.285.3[农业科学—兽医学]