序贯疗法对化疗致POF大鼠血清性激素的影响  

Effect of Yin and Yang Sequential Therapy of the Cycle Time on Rats Serum Sex Hormones of Premature Ovarian Failure due to Chemotherapy

作　　者：李莎[1] 薛瑞霞[1] 苏燕[1] 崔晓萍[2] 

机构地区：[1]陕西中医学院 [2]陕西中医学院附属医院

出　　处：《山西中医》2014年第5期51-52,共2页Shanxi Journal of Traditional Chinese Medicine

基　　金：陕西省中医管理局中标项目(编号:jc11)

摘　　要：目的:观察循期阴阳序贯疗法对化疗所致的卵巢早衰(POF)大鼠血清性激素水平的影响。方法:选取动情周期规律的SD雌性大鼠,运用环磷酰胺腹腔注射建立POF大鼠模型,随机分为空白组、模型组、左归丸组、右归丸组、循期阴阳序贯低、中、高剂量组、西药组,连续灌胃20天。采用放免法检测外周血FSH、LH及E2含量。结果:循期阴阳序贯低、中、高剂量组及西药组的大鼠血清FSH、LH水平降低,E2水平升高,与模型组比较,均有显著意义,其中高剂量组及西药组有极显著意义(P<0.01)。结论:循期阴阳序贯疗法能缓解POF大鼠外周血FSH及LH的升高,并显著增加E2含量,说明其对化疗致POF大鼠的卵巢有一定的保护作用。Objective ： To observe the effect of yin and yang sequential therapy of cycle time on the level changes of rats serum sex hormones of premature ovarian failure （POF） due to chemotherapy,so as to provide the effective solutions and experimental basis for POF prevention with Traditional Chinese Medicine. Methods：To se- lect SD female rats with estrous cycle regularity;to establish a rat model of POF by intraperitoneal injection of cy- clophosphamide. These rats were randomly divided into blank group, model group, zuoguiwan group, youguiwan group, low, middle and high dose groups using yin and yang sequence of cycle time ~ western medicine group, and gavaged continuously for 20 days. By radioimmunoassay to detect the cotents of peripheral blood FSH, LH and E2. Results ： Compared with the model group, in low, middle and high dose groups Using yin and yang sequence of cy- cle time and western medicine group, the levels of rats serum sex hormones were significant ; among them, high- dose group and western medicine group had highly significant （ P 〈 0.01 ）. Conclusion ： yin and yang sequential therapy of the tycle time can ease off the increasing of rat peripheral blood FSH and LH of POF, and increase E2 levels significantly. This indicats that it has a certain protective effect for rat with POF due to chemotherapy.

关 键 词：卵巢早衰 大鼠 性激素 实验研究 

分 类 号：R458.7[医药卫生—治疗学]