湖南辣椒疫霉病菌ITS的扩增和序列分析  

Amplification of ITS Sequence and Sequence Analysis for Phytophthora capsici in Hunan Province

作　　者：陈冰[1,2,3] 张卓 张德咏 谭新球 刘二明[1] 刘勇 

机构地区：[1]湖南农业大学植物保护学院,湖南长沙410128 [2]园艺作物病虫害治理湖南省重点实验室,湖南长沙410125 [3]湖南省植物保护研究所,湖南长沙410125

出　　处：《湖南农业科学》2014年第4期59-61,共3页Hunan Agricultural Sciences

基　　金："十二五"国家科技支撑计划项目(2012BAD19B06);公益性行业(农业)科研专项目(201003004);现代农业产业技术体系岗位专家项目(CARS-25-B-05)

摘　　要：利用PCR技术,对湖南长沙、娄底、郴州、岳阳、湘西、张家界等地发病辣椒样品分离鉴定22个辣椒疫霉病菌的ITS序列进行扩增,测序获得大小约870 bp的DNA片段。通过与报道的辣椒疫霉病菌的ITS序列进行比对,构建系统发育进化树,结合AFLP分析,表明湖南省内不同供试菌株间的遗传相似性与地理来源没有直接相关性。Twenty-two strains ofPhytophthora capsici were isolated and identiifed from the infected samples of peppers that collected from Changsha, Loudi, Chenzhou, Yueyang, Xiangxi and Zhangjiajie cities of Hunan province. Their ITS regions were amplified by PCR, and then the products were sequenced with size of about 870 bp. A phylogenetic tree of those ITS sequences was constructed using neighbor-joint methods, and with the AFLP analysis, it showed that there was no correlation between DNA polymorphism and geographical origins among the 22 strains.

关 键 词：辣椒疫霉病菌 ITS序列 系统发育树 AFLP 

分 类 号：S436.418[农业科学—农业昆虫与害虫防治]