乙型肝炎患者血清乙型肝炎病毒外膜大蛋白LHBs的检测分析  

Detection of hepatitis B virus large surface protein in the sera of HBV-infected patients

作　　者：刘键[1] 蒋英[2] 吴正林[1] 黄烈[1] 庄鹏[1] 

机构地区：[1]深圳市第四人民医院检验医学部,广东深圳518033 [2]深圳市福田区慢性病防治院检验科

出　　处：《实用预防医学》2014年第5期607-609,共3页Practical Preventive Medicine

基　　金：深圳市福田区公益性科研项目深圳市科技局项目(编号FTWS081)

摘　　要：目的探讨乙型肝炎病毒表面大蛋白(LHBs)检测对乙型肝炎诊断的实验价值。方法采用酶联免疫吸附实验(ELISA)和荧光定量PCR法对1 049例HBsAg阳性及694例HBeAg阴性慢性乙肝患者血清标本进行HBV DNA和LHBs检测。结果 HBsAg阳性患者中HBV DNA阳性率为66.12%(653/1 049),LHBs阳性率为68.18%(677/1 049),二者差异无统计学意义(χ2=1.18,P>0.05);LHBs含量与HBV DNA拷贝数对数值呈正相关性;HBeAg阴性慢性乙肝患者中HBV DNA与LHBs检出阳性率分别为43.80%(304/694)和51.73%(359/694),二者差异有统计学意义(χ2=8.74,P<0.01)。结论 LHBs能反映乙型肝炎病毒复制情况;对HBeAg阴性慢性乙肝患者而言,LHBs更能反映体内HBV感染和复制状态。Objective To explore the experimental value of hepatitis B virus large surface protein （LHBs） in the diagnosis of hepatitis B. Methods Enzyme- linked immunosorbent assay （ELISA） and fluorescence- based quantitative PCR were used for detection of HBV DNA and LHBs in 1,049 HBsAg - tx）sitive and 694 HBeAg - negative patients with chronic hepatitis B. Results The positive rates of HBV DNA and LHBs in HtKsAg- positive patients were 66.12 % （653/1,049） and 68.18 % （677/1,049） respectively, and no statistically significant difference was found between them （X^2-1.18, P 〉 0.05 ）. The serum LHIKs concentration was positiwe correlated with the logarithmic value of HBV DNA copies among 653 HBV DNA positive pa- tients. The positive rates of HI3V DNA and LHBs in HBeAg negative patients were 43.80 % （304/694） and 51.73 % （359/ 694） respectively, with statistically significant differences （X^2 -8.74, P〈 0.01）. Conclusions LHtKs can reflect the repli- cation of HBV. Compared with HBV DNA, LHI3s is a better biological marker to reflect the infection and duplication of HBV among HBeAg- negative chronic hepatitis t3 patients.

关 键 词：乙型肝炎 乙型肝炎病毒外膜大蛋白 HBV DNA 

分 类 号：R512.62[医药卫生—内科学]